BLADE team

BacteriaL Adaptation, Diversity and Engineering

A team dedicated to

The bacterial physiology

The research activities of the BLADE team are in the field of bacterial physiology and aim to better understand the mechanisms of adaptation of bacteria to their environment to better control their use. The team aims 1) to acquire fundamental and mechanistic knowledge on the functioning of bacteria and 2) to increase their performance in biotechnology through engineering strategies that meet the challenges of the bio-economy. The team is particularly interested in two model bacteria, Lactococcus lactis and Escherichia coli, whose fields of application are the food industry, human health and white biotechnology.

The scientific approach of the team uses the approaches and methodologies of systems biology. It consists in characterizing the different levels of cellular processes (genes, mRNAs, proteins and metabolites) using "omics" tools, which are then integrated by mathematical models in order to acquire a systemic and hierarchical vision of all the regulations at play. Bacteria are studied by subjecting them to genetic (mutants) or environmental perturbations (stress, change of carbon source, nutrient depletion, etc.), alone or in interaction with other bacteria or heterogeneous subpopulations.

The team's topics

  • ANR
  • TWB
  • Région Occitanie
  • TTT
  • 3Bcar 
  • Lesaffre
  • Pedro Gomes. Exploration du potentiel santé de souches de Lactococcus lactis dans le dialogue bidirectionnel avec l’hôte. Direction H. Eutamene [Toxalim] et M. Cocaign-Bousquet [TBI]
  • Fan Chen. Rôle de séquences régulatrices dans la régulation de l’expression génique chez Escherichia coli. Direction L. Girbal [TBI] et S. Nouaille [TBI]
  • Charlotte Roux. Rôle de la polyadénylation des ARN dans la régulation du métabolisme de Escherichia coli. Direction L. Girbal [TBI] et E. Hajnsdorf [IBPC]

Thèses soutenues depuis 2019 

  • Kevin Debatisse. Reprogrammation de la spécificité de recombinaison d’une intégrase phagique. Direction P. Le Bourgeois [TBI]  
  • Manon Barthe. L’hétérogénéité métabolique dans la capacité adaptative de la bactérie Escherichia coli. Direction M. Cocaign-Bousquet [TBI] et B. Enjalbert [TBI] 
  • Marie-Aurore Caillaud. Dynamique, stabilité et robustesse d’un ferment autochtone. Direction M.L. Daveran-Mingot [TBI] et H. Tormo [EI Purpan] 
  • Etienne T. Analyse intégrative de la coordination entre stabilité des ARNm et physiologie cellulaire chez Escherichia coli. Direction D. Ropers [INRIA] et M. Cocaign-Bousquet [TBI]. 
  • Nguyen H. Régulation de la traduction des ARNm chez Escherichia coli. Direction M. Cocaign-Bousquet [TBI] et L. Girbal [TBI].