GeT-Biochips platform

We know that your samples are precious, it is possible to make quality controls on small quantities (50pg/µL minimum) and small volumes of samples (1,5µL minimum). We perform quantitative and qualitative nucleic acid assays for DNA, RNA, single stranded DNA, protein assays, fluorescently labeled DNA assays.

We advise you on the interpretation of your results.

The ND2000 nanodrop is available for use by TBI staff in autonomy after training. It can be booked at the address : https://get-biopuces.insa-toulouse.fr/booking/login.php

To verify the integrity of your DNAs and RNAs, we can perform assays on the Bioanalyzer (Agilent) or the Qubit (Thermofisher).

Do not hesitate to contact us for more information: contact us.

We propose to carry out your transcriptomic studies using DNA chips (Agilent or Affymetrix). We advise you on the implementation of the experimental plan (number of replicates, extraction method, ...etc). Then, we carry out the steps of marking, hybridization, washing and scanning of the chips.

Data analysis is done by the platform staff.

Platform equipment:

• Deposit robot: Qarray mini Genetix
• GeneAmp PCR system 9700 Applied Biosystem
• Agilent hybridization oven
• Scanner MS200 Tecan
• Scanner 900 Innopsys
• Affymetrix hybridization oven
• Station de lavage affymetrix Fluidics Station 450
• Genechip Scanner Affymetrix

We offer the necessary tools for DNA and RNA sequencing. We advise you on the implementation of the experimental plan (number of replicates, extraction method, ...etc)        

We can handle the ribodepletion of your RNAs or the selection of polyA RNAs if required.

We prepare all types of libraries (Thermofisher, Illumina, Oxford Nanopore) from DNA, RNA or PCR products.

The sequencing is then performed on the most suitable machine:

• Ion GeneStudio S5 system (ThermoFisher) pour les petits génomes (bactéries, levures, fragments PCR, métagénomique 16S, 18S, ITS) en single-reads (100bp, 200bp et 400bp)
• Ion GeneStudio S5 system (ThermoFisher) for small genomes (bacteria, yeast, PCR fragments, 16S, 18S, ITS metagenomics) in single-reads (100bp, 200bp and 400bp)
• MinION (Oxford Nanopore) for long fragment DNA sequencing of small organisms (bacteria, yeast, algae), in single reads 8-10kb
• Access to Illumina NovaSeq and NextSeq sequencers in partnership with other sites in pair-end 2X150bp, 2X75bp

We also support you in the analysis of your data.

Among the available applications, we propose:

• De novo sequencing
• Re-sequencing
• Ampliseq (PCR fragment sequencing)
• Sequencing of fosmids
• RNAseq
• Metagenomics V1-2, V3-4 or all variable regions (Illumina Miseq, Ion Torrent S5)

We are also a microbiology ambassador within GeT : contact us for your projects

L’analyse des données est l’une des parties les plus fastidieuses dans un projet. Grâce à son équipe pluridisciplinaire, la plateforme vous propose un accompagnement personnalisé ainsi que des solutions adaptées à vos projets qu’ils soient en transcriptomique (expression différentielle, enrichissement fonctionnel, single cell) ou en génomique (analyse de variants, assemblage de fosmides, analyses métagénomiques ciblée avec rANOMALY)

Les données peuvent provenir :

• d’une prestation en service de la plateforme
• d’une prestation d’une autre plateforme
• d’un projet de collaboration

Nous proposons également des formations pour des sociétés ou personnels désireux de s’initier à l’analyse bio-informatique des données : contact us.