Some equipment

Pilot

  • Epurogas: gas wash (4 m high, 50 cm diameter)
  • Flow in a gas-liquid pocket pipe (4.5 m long, diameter 40 mm) with synchronisation of 3 cameras (PIV+ombroscopy)
  • Bubble plume (2 m quasi 2D)
  • DAF dissolved air flotation (1 m3/h) for the capture of algae, flocs, drops or nanoparticles
  • Bubble or drop column (2 m, diameter 50 cm)
  • Standard 70l stirred tank
  • Coagulation / flocculation / decantation (40l quasi 2D)

Measuring system

  • Optical tweezers (500mW laser, Thorlabs inverted microscope + microscope)
  • Particle velocity tracking (PIV): dual 532 nm laser 2×30 mJ + 4 Dantec cameras
  • Photron SA3 high-speed camera, Phantom Mini, PCO1200 (speed > 1000 Hz)
  • Laser-induced fluorescence measurement (PLIF): 2x 200 mJ dual laser
  •  Optical drop and bubble size and velocity sensor (A2S)
  • 8 "Slow" cameras < 300 Hz for umbroscopy, colorimetry, general microscopic monitoring
  • 2 In-house epifluorescence microscope (inverted with 4 fluorescence class cubes) 0.5 micron/pixel
  • Home-made transmission fluorescence microscope
  • Particle size measurement (Laser Scattering, Malvern Spraytec, DLS, Nanotrac Microtrac)

Model experience

  • Shear flow chamber (100 microns thick) + microscope camera
  • Micro-focusing of particles / micro-organisms in a micrometric square tube
  • Particle / microorganism deposition chamber on micro-sieve (2-5 micron holes) + microscope
  • Hele Shaw diffusion cell (quasi 2D thickness of 2 to 5 mm)
  • Isolated rising bubble (0.5 mm to 5 mm bubble, wake transfer, particle capture).
  • Flotation chamber (quasi 2D thickness 10 mm) with white water

Digital tools

  • Fluent-Ansys fluid mechanics software suite (finite volumes)
  • Matlab (Toolbox Image Processing, Opimization)
  • Mechanical design Autodesk inventor
  • Comsol multiphysics simulation (finite elements)
  • ADENON home-made code for dynamic modelling of bioreactors (Matlab )
TBI, Toulouse Biotechnology Institute
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