Topic "Biological variability and cellular adaptation"
A few words
Genetic, epigenetic and gene expression variability
Genetic, epigenetic and gene expression variability (the so-called expression noise) are generally considered independently in their relationship with phenotypic variation. However, they appear to be intrinsically interconnected and influence it in combination. In this topic, we ask whether these three sources of biological variability influence each other, and whether their interplay modulates phenotypic variability and cellular adaptation in the yeast Saccharomyces cerevisiae. Through single-cell approaches, we focus on various genomic and epigenomic phenomena (homologous recombination rate and transgene stability, epigenetic silencing...) and on various phenotypic traits related to biotechnological issues, such stress resistance, single-cell growth rate, cellular aging…
Precompetitive project “NoHighRec” at Toulouse White Biotechnology (TWB) from October 2020 to September 2022. Full title: Towards continuous elimination of high-recombination cells to stabilize industrial strains. In line with our previous results characterizing the cell-to-cell heterogeneity in the rate of homologous recombination in S. cerevisiae (Liu et al, Front Genet, 2019), this project aims at (1) characterizing the genetic instability of high-recombination (« high-rec ») cells compared to « low-rec cells », particularly at the level of transgenes conferring production features, and (2) developing a synthetic gene network capable of continuously removing high-rec cells to avoid loss of production by this source of genetic instability.