Stéphane GUILLOUET

GUILLOUET Stéphane

Stéphane GUILLOUET

Professor in Metabolic and Biochemical Engineering at INSA Toulouse

Equipe Metasys

Actual position and research subject

Research projects in metabolic and biochemical engineering :

  • Gas fermentation : Microbial production of biomolecules from CO2 as sole carbon source.
  • Production of various biomolecules from other renewable materials.

Education and Diploma

Teaching

Over 200 hours of teaching in Microbial Metabolism, Microbial Kinetics and Fermentation strategy at INSA Toulouse (M1 and M2 levels)

Others

  • Supervised or co-supervised: 18 PhD students and 10 post-doct
  • Contribution to research projects: Coordinator and WP leader of national and international projects

List of selected publications

  • Weickardt, E. Lombard, A. Zhang, L. Blank, S.E. Guillouet. Comparative characterisation of autotrophic and heterotrophic isopropanol formation by Cupriavidus necator in shake flasks. Journal of Biotechnology, 2025, 403 1–8. https://doi.org/10.1016/j.jbiotec.2025.03.011
  • Di Bisceglie, J. García Navarro, E. Lombard, R. Kratzer, R. Kourist, S.E. Guillouet. Conceptual approach for aerobic autotrophic gas cultivation in shake flasks: Overcoming the inhibitory effects of oxygen in Cupriavidus necator. Biotechnology Journal, 2025; 20:e202400641. https://doi.org/10.1002/biot.202400641
  • Gelain, J.W. Yeoh, G. S. Hossain, S. Alfenore, S. Guillouet, H. Ling, C. L. Poh, N. Gorret, J. L. Foo. Identifcation and monitoring of cell heterogeneity from plasmid recombination during limonene production. Applied Microbiology and Biotechnology 2025. 109:4. https://doi.org/10.1007/s00253-024-13273-5
  • Buron-Moles, V. Vandenbossche, N. Gorret, L. Santonja-Blasco, T. Gonzalez-Aranda, X. Cameleyre, S. Guillouet. Biodegradation of pre-treated low-density polyethylene (LDPE) by Yarrowia lipolytica determined by oxidation and molecular weight reduction. Polymer Degradation and Stability 236, 2025, 111292 https://doi.org/10.1016/j.polymdegradstab.2025.111292
  • Joris, E. Lombard, A. Paillet, G. Navarro, S.E. Guillouet, N. Gorret. Recycling potential of Cupriavidus necator for life support in space: Production of SCPs from volatile fatty acid and urea mixture. Journal of Biotechnology 2024. 396 18–27. https://doi.org/10.1016/j.jbiotec.2024.10.001
  • Ismail, S., Giacinti, G., Delagado Raynaud, C., Cameleyre, X., Alfenore, S., Guillouet, S. , Gorret, N. Impact of the environmental parameters on single cell protein production and composition by Cupriavidus necator. J. Biotechnol. 2024. 388 83–95. https://doi.org/10.1016/j.jbiotec.2024.04.009
  • Joris, P., Navarro, G., Paillet, A., Lombard, E. ,Gorret, N., Guillouet, S.. Assessing the Recycling Potential of Cupriavidus necator for Space Travel: Production of SCPs from Organic Waste, International Conference on Environmental Systems 2023 https://hdl.handle.net/2346/94656
  • Sydow, A.; Becker, L.; Lombard, E.; Ulber, R.; Guillouet, S.E.; Holtmann, D. Autotrophic Production of the Sesquiterpene α-Humulene with Cupriavidus necator in a Controlled Bioreactor. Bioengineering 2023, 10, 1194. https://doi.org/10.3390/ bioengineering10101194
  • Boy , J. Lesage, S. Alfenore, N. Gorret, S E. Guillouet, 2023 Comparison of plasmid stabilization systems during heterologous isopropanol production in fed-batch bioreactor. J.Biotechnol. doi.org/10.1016/j.jbiotec.2023.02.011
  • Boy , J. Lesage, S. Alfenore, S E. Guillouet, N. Gorret, 2022 Study of plasmid-based expression level heterogeneity under plasmid-curing like conditions in Cupriavidus necator. J Biotechnol 345 : 17-29. https://doi.org/10.1016/j.jbiotech.2021.12.015.
  • Boy , J. Lesage, S. Alfenore, S E. Guillouet, N. Gorret 2021.Investigation of the robustness of Cupriavidus necator engineered strains during fed‑batch cultures. AMB Express 11 : 151. https://doi.org/10.1186/s13568-021-01307-4
  • Garrigues, T. D. Do, C. Bideaux, S. E. Guillouet, I. Meynial-Salles. 2021 Insights into Clostridium tetani: From genome to bioreactors. Biotechnol Adv. https://doi.org/10.1016/j.biotechadv.2021.107781
  • Lesage, A. Timoumi, S. Cenard , E. Lombard, H. L.T. Lee , S.E. Guillouet , N Gorret 2021 Accelerostat study in conventional and microfluidic bioreactors to assess the key role of residual glucose in the dimorphic transition of Yarrowia lipolytica in response to environmental stimuli. New Biotechnol 64 : 37–45. https://doi.org/10.1016/j.nbt.2021.05.004
  • Boy , J. Lesage, S. Alfenore, N. Gorret, S E. Guillouet. 2020. Plasmid expression level heterogeneity monitoring via heterologous eGFP production at the single-cell level in Cupriavidus necator. Appl Microbiol Biotechnol. https://doi.org/10.1007/s00253-020-10616-w.
  • Garrigues L., Maignien L., Lombard E., Singh J., E. Guillouet 2019 Isopropanol production from carbon dioxide in Cupriavidus necator in a pressurized bioreactor. New Biotechnol. 56, 16-20. https://doi.org/10.1016/j.nbt.2019.11.005
  • Crépin L, Barthe M, Leray F, Guillouet SE. 2018 . Alka(e)ne synthesis in Cupriavidus necator boosted by the expression of endogenous and heterologous ferredoxin-ferredoxin reductase systems. Biotechnol Bioeng.115(10):2576-2584. doi: 10.1002/bit.26805.
  • Sagnak, S. Cochot, C. Molina-Jouve, J.-M. Nicaud and S. E. Guillouet. 2017. Journal Biotechnology. Modulation of the Glycerol Phosphate availability led to concomitant reduction in the citric acid excretion and increase in lipid content and yield in Yarrowia lipolytica. doi.org/10.1016/j.jbiotec.2017.11.001.
  • Marc, E. Grousseau, E. Lombard, A. J. Sinskey, N. Gorret and S. E. Guillouet. 2017. Metabolic Engineering. Over expression of GroESL in Cupriavidus necator for heterotrophic and autotrophic isopropanol production. doi.org/10.1016/j.ymben.2017.05.007
  • Crepin L, Lombard E, Guillouet SE . 2016. Metabolic engineering of Cupriavidus necator for heterotrophic and autotrophic alka(e)ne production. Metabolic Engineering 37:92-101 doi:10.1016/j.ymben.2016.05.002
  • Grunwald S, Mottet A, Grousseau E, Plassmeier JK, Popović MK, Uribelarrea J-L, Gorret N, Guillouet SE, Sinskey A. 2015. Kinetic and stoichiometric characterization of organoautotrophic growth of Ralstonia eutropha on formic acid in fed-batch and continuous cultures. Microbial Biotechnology 8:155-163.

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