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Professor in Metabolic and Biochemical Engineering at INSA Toulouse

Actual position and research subject

Head of the Research team Fermentation Advances and Microbial Engineering at TBI

Research projects in metabolic and biochemical engineering :

  • Gas fermentation : Microbial production of biomolecules from CO2 as sole carbon source.
  • Production of various biomolecules from other renewable materials.

Education and Diploma


Over 200 hours of teaching in Microbial Metabolism, Microbial Kinetics and Fermentation strategy at INSA Toulouse (M1 and M2 levels)


  • Supervised or co-supervised: 18 PhD students and 10 post-doct
  • Contribution to research projects: Coordinator and WP leader of national and international projects

List of selected publications

  • Boy , J. Lesage, S. Alfenore, S E. Guillouet, N. Gorret, 2022 Study of plasmid-based expression level heterogeneity under plasmid-curing like conditions in Cupriavidus necator. J Biotechnol 345 : 17-29.
  • C. Boy , J. Lesage, S. Alfenore, S E. Guillouet, N. Gorret 2021.Investigation of the robustness of Cupriavidus necator engineered strains during fed‑batch cultures. AMB Express 11 : 151.
  • Garrigues, T. D. Do, C. Bideaux, S. E. Guillouet, I. Meynial-Salles. 2021 Insights into Clostridium tetani: From genome to bioreactors. Biotechnol Adv.
  • Lesage, A. Timoumi, S. Cenard , E. Lombard, H. L.T. Lee , S.E. Guillouet , N Gorret 2021 Accelerostat study in conventional and microfluidic bioreactors to assess the key role of residual glucose in the dimorphic transition of Yarrowia lipolytica in response to environmental stimuli. New Biotechnol 64 : 37–45.
  • Boy , J. Lesage, S. Alfenore, N. Gorret, S E. Guillouet. 2020. Plasmid expression level heterogeneity monitoring via heterologous eGFP production at the single-cell level in Cupriavidus necator. Appl Microbiol Biotechnol.
  • Garrigues L., Maignien L., Lombard E., Singh J., E. Guillouet 2019 Isopropanol production from carbon dioxide in Cupriavidus necator in a pressurized bioreactor. New Biotechnol. 56, 16-20.
  • Crépin L, Barthe M, Leray F, Guillouet SE. 2018 . Alka(e)ne synthesis in Cupriavidus necator boosted by the expression of endogenous and heterologous ferredoxin-ferredoxin reductase systems. Biotechnol Bioeng.115(10):2576-2584. doi: 10.1002/bit.26805.
  • Sagnak, S. Cochot, C. Molina-Jouve, J.-M. Nicaud and S. E. Guillouet. 2017. Journal Biotechnology. Modulation of the Glycerol Phosphate availability led to concomitant reduction in the citric acid excretion and increase in lipid content and yield in Yarrowia lipolytica.
  • Marc, E. Grousseau, E. Lombard, A. J. Sinskey, N. Gorret and S. E. Guillouet. 2017. Metabolic Engineering. Over expression of GroESL in Cupriavidus necator for heterotrophic and autotrophic isopropanol production.
  • Crepin L, Lombard E, Guillouet SE . 2016. Metabolic engineering of Cupriavidus necator for heterotrophic and autotrophic alka(e)ne production. Metabolic Engineering 37:92-101 doi:10.1016/j.ymben.2016.05.002
  • Grunwald S, Mottet A, Grousseau E, Plassmeier JK, Popović MK, Uribelarrea J-L, Gorret N, Guillouet SE, Sinskey A. 2015. Kinetic and stoichiometric characterization of organoautotrophic growth of Ralstonia eutropha on formic acid in fed-batch and continuous cultures. Microbial Biotechnology 8:155-163.
  • Richard L, Guillouet SE, Uribelarrea JL. 2014. Quantification of the transient and long-term response of Saccharomyces cerevisiae to carbon dioxide stresses of various intensities. Process Biochemistry 49:1808-1818.
  • Ochoa-Estopier A, Guillouet SE. 2014. D-stat culture for studying the metabolic shifts from oxidative metabolism to lipid accumulation and citric acid production in Yarrowia lipolytica. Journal of Biotechnology 170:35-41.
  • Grousseau E, Lu JN, Gorret N, Guillouet SE, Sinskey AJ. 2014. Isopropanol production with engineered Cupriavidus necator as bioproduction platform. Applied Microbiology and Biotechnology 98:4277-4290.
  • Pagliardini J, Hubmann G, Alfenore S, Nevoigt E, Bideaux C, Guillouet SE. The metabolic costs of improving ethanol yield by reducing glycerol formation capacity under anaerobic conditions in Saccharomyces cerevisiae. Microbial Cell Factories 12.
  • Ochoa-Estopier A, Lesage J, Gorret N, Guillouet SE. 2011. Kinetic analysis of a Saccharomyces cerevisiae strain adapted for improved growth on glycerol: Implications for the development of yeast bioprocesses on glycerol. Bioresource Technology 102:1521-1527.
  • Hubmann G, Guillouet S, Nevoigt E. 2011. Gpd1 and Gpd2 Fine-Tuning for Sustainable Reduction of Glycerol Formation in Saccharomyces cerevisiae. Applied and Environmental Microbiology 77:5857-5867.
  • Pagliardini J, Hubmann G, Bideaux C, Alfenore S, Nevoigt E, Guillouet SE. 2010. Quantitative evaluation of yeast’s requirement for glycerol formation in very high ethanol performance fed-batch process. Microbial Cell Factories 9.
  • Guillouet S, Rodal AA, An GH, Gorret N, Lessard PA, Sinskey AJ. 2001. Metabolic redirection of carbon flow toward isoleucine by expressing a catabolic threonine dehydratase in a threonine-overproducing Corynebacterium glutamicum. Applied Microbiology and Biotechnology 57:667-673.
  • S. Guillouet and J.M. Engasser. 1996. Appl. Microbiol. Biotechnol. 46 : 291-296. Growth of Corynebacterium glutamicum in ammonium and potassium-limited continuous cultures under high osmotic pressure.