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Stéphanie HEUX

Stéphanie Heux

Stéphanie HEUX

Director of research INRAE
METASYS Team leader

Actual position and research subject

I am leading the Metasys team at Toulouse Biotechnology Institute (TBI, former LISBP). My research aimed at developing innovative and original biotechnological processes using microorganisms as cell factory for upgrading renewable carbon resource for bioproducts. I go beyond the natural by designing new synthetic metabolic networks which can perform particular functions that does not exist in nature. I then analyze their functioning at the cellular level using genome scale models, transcriptomics metabolomics and fluxomics. This knowledge helps me to optimize the synthetic cell factories but also to reach a deeper understanding of how life works. The renewable carbon resource, I am interested in, is methanol which can produced from the greenhouse gas CO2 through renewable photo and electrochemical processes, thus helping to mitigate global warming. My work focuses on both natural (in Bacillus methanolicus) and synthetic (in Escherichia coli) methylotrophy, a process which represents a real metabolic challenge. My goal is to get the full picture of what is necessary for methylotrophic growth in natural and synthetic methylotrophs (i.e. spatial organization, regulation and metabolism) and apply this fundamental understanding to develop microbial cell factories for the sustainable production of value-added products from methanol.

Education and Diploma

Training & Teaching

  • Post-Doc students: 6
  • PhD students: 1
  • Master 2 students: 9
  • Others: 56 (IGEM teams, students in BTS, DUT, Master 1 and engineer)
  • Teaching: 26 hours in system biotechnology

Others

  • European Federation of Biotechnology (EFB), Secretary of the Microbial Biotechnology Division
  • Member of the scientific committee of the TRANSFORM department of INRAE
  • Member of the scientific committee of TBI
  • In charge of scientific animation within the MICA department of INRAE

Others

  • European Federation of Biotechnology (EFB), Secretary of the Microbial Biotechnology Division
  • Member of the scientific committee of the TRANSFORM department of INRAE
  • Member of the scientific committee of TBI
  • In charge of scientific animation within the MICA department of INRAE

List of selected publications

  1. Lessmeier, L., J. Pfeifenschneider, M. Carnicer, S. Heux, J. C. Portais and V. F. Wendisch (2015). “Production of carbon-13-labeled cadaverine by engineered Corynebacterium glutamicum using carbon-13-labeled methanol as co-substrate.” Appl Microbiol Biotechnol 99(23): 10163-10176 DOI: 10.1007/s00253-015-6906-5.
  2. Muller, J. E. N., F. Meyer, B. Litsanov, P. Kiefer, E. Potthoff, S. Heux, W. J. Quax, V. F. Wendisch, T. Brautaset, J. C. Portais and J. A. Vorholt (2015). “Engineering Escherichia coli for methanol conversion.” Metabolic Engineering 28: 190-201 DOI: 10.1016/j.ymben.2014.12.008.
  3. Carnicer, M., G. Vieira, T. Brautaset, J. C. Portais and S. Heux (2016). “Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus.” Microbial Cell Factories 15 DOI: 10.1186/s12934-016-0483-x.
  4. Heux, S., C. Berges, P. Millard, J. C. Portais and F. Letisse (2017). “Recent advances in high-throughput 13C-fluxomics.” Curr Opin Biotechnol 43: 104-109 DOI: 10.1016/j.copbio.2016.10.010.
  5. Cottret, L., C. Frainay, M. Chazalviel, F. Cabanettes, Y. Gloaguen, E. Camenen, B. Merlet, S. Heux, J. C. Portais, N. Poupin, F. Vinson and F. Jourdan (2018). “MetExplore: collaborative edition and exploration of metabolic networks.” Nucleic Acids Res 46(W1): W495-W502 DOI: 10.1093/nar/gky301.
  6. De Simone, A., C. M. Vicente, C. Peiro, L. Gales, F. Bellvert, B. Enjalbert and S. Heux (2020). “Mixing and matching methylotrophic enzymes to design a novel methanol utilization pathway in E. coli.” 61: 315-325 DOI: 10.1016/j.ymben.2020.07.005.
  7. Delepine, B., M. G. Lopez, M. Carnicer, C. M. Vicente, V. F. Wendisch and S. Heux (2020). “Charting the Metabolic Landscape of the Facultative Methylotroph Bacillus methanolicus.” mSystems 5(5) DOI: 10.1128/mSystems.00745-20.
  8. Hennig, G., C. Haupka, L. F. Brito, C. Ruckert, E. Cahoreau, S. Heux and V. F. Wendisch (2020). “Methanol-Essential Growth of Corynebacterium glutamicum: Adaptive Laboratory Evolution Overcomes Limitation due to Methanethiol Assimilation Pathway.” Int J Mol Sci 21(10) DOI: 10.3390/ijms21103617.
  9. Millard, P., U. Schmitt, P. Kiefer, J. A. Vorholt, S. Heux and J. C. Portais (2020). “ScalaFlux: A scalable approach to quantify fluxes in metabolic subnetworks.” PLoS Comput Biol 16(4): e1007799 DOI: 10.1371/journal.pcbi.1007799.
  10. Bergès, C., E. Cahoreau, P. Millard, B. Enjalbert, M. Dinclaux, M. Heuillet, H. Kulyk, L. Gales, N. Butin, M. Chazalviel, T. Palama, M. Guionnet, S. Sokol, L. Peyriga, F. Bellvert, S. Heux and J.-C. Portais (2021). “Exploring the Glucose Fluxotype of the E. coli y-ome Using High-Resolution Fluxomics.” Metabolites 11(5): 271 DOI: 10.3390/metabo11050271

 

To see all my publication please visit:

https://orcid.org/my-orcid?orcid=0000-0003-1312-3002 or

https://hal.archives-ouvertes.fr/search/index/?q=heux-stephanie