The the 23rd of september 2022 at 9:30 am
room 401 of Toulouse Biotechnology Institute
Title
“A contribution to the study of aerobic granulation and ALE production: effects of high organic loading rates, and different COD/N ratio“
Jury composition:
- Mme. Sylvie GILLOT, Directrice de recherche, REVERSAAL, Lyon – Rapporteuse
- M. Jean-Philippe DELGENES, Directeur de recherche, LBE, Narbonne – Rapporteur
- Mme. Elisabeth NEUHAUSER, Professeure, LBAE, Auch – Examinatrice
- M. Nicolas DERLON, Directeur de recherche, EAWAG, Zürich – Examinateur
- M. Thibaut SAUR, Ingénieur innovation, SUEZ, France – Invité
- M. Etienne PAUL, Professeur, TBI, Toulouse – Directeur de Thèse
- Mme. Yolaine BESSIERE, Maitresse de conférence, TBI, Toulouse – Co-directrice de thèse
Aerobic granular sludge allows multiple reactions and fast-settling among the same tank (Morgenroth et al., 1997). Those elements explain the fast spreading of granular sludge processes for domestic and  industrial  wastewater  treatment  (Pronk  et  al.,  2015).    In  addition,  the  potential  valorization  of  ALE (alginate-like  exopolymers)  seems  an  interesting  route  for  circular  economy  of  wastewaters  (van Leeuwen et al., 2018).
This thesis was interested in studying the possibility to valorize ALE from particular (industrial), highly loaded wastewaters. The impact of different operating conditions on granular sludge formation were studied as well as their consequences in terms of ALE production. In particular, the conditions studied were contrasted COD/N, high COD/P and high organic loading rate. All along the thesis a complementary  modelling  approach  was  used  to  gain  insight  in  the  biological  mechanisms  and competitions taking place under the applied conditions.
Doing such allowed to observe that  a low nitrogen loading rate led to fast granulation and thus ALE formation.  On  the  contrary,  a  high  nitrogen  loading  rate  led  to  a  loss  of  granulation,  meaning  an absence of structural EPS. The phenomenon behind this granulation inability were studied in depth.   
Moreover,  organic  loading  rate  modifications  led  to  the  observation  of  a  correlation  between  the kinetic biological constants and the ALE production for the first time.
This  thesis  brings  new  elements  to  go  towards  the  valorization  of  high  added-value  products  in wastewater treatment plants.
 
				



