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Régis FAURE

Régis Faure

Régis FAURE

CNRS senior research scientist

Actual position and research subject

My research interests focus on biomolecular engineering, combining chemical synthesis and protein engineering approaches, to design evolved and/or artificial enzymatic catalysts for glycosylation (enzyme catalysis, bioorganic chemistry & biomolecular engineering).

After a first degree in Physical Chemistry (University of Toulouse, 1998) and a PhD in Biological Chemistry (University of Grenoble, 2004), in the field of chemo-enzymatic glycosynthesis, I spent almost two years as a postdoctoral fellow in the field of glycochemistry in the Department of Chemistry at UQAM. Since joining the CNRS as a tenured researcher in TBI (2007), I pursued the development of my skills in organic synthesis, while acquiring new knowledge and practical knowhow in the fields of protein biochemistry, enzyme engineering and high-throughput screening. I supply vital in-house expertise in the field of (glyco)chemistry, supporting research focused on enzyme discovery through the design of ad hoc substrates for easy specific detection of enzymatic activities. I also provide strong input in the area of enzymatic (glyco)synthesis, using carbohydrate active enzymes (transglycosylases from evolved glycoside hydrolases, esterases, etc.) and work on development of novel enzymes for de novo synthesis of carbohydrates. Most recently, I have shifted a part of my research activity towards the redesign of enzyme active sites, also targeting protein glycosylation as an applied aim. This research calls upon my knowledge of chemistry and uses non-canonical amino acids to extend the chemical functionalities of naturally occurring enzymes.

Education and Diploma

Others

  • Supervised or co-supervised: 5 PhD students and 10 postdoctoral fellows
  • Stays as a visiting scientist with A. Planas, IQS Barcelona, Spain (2015, 7 months); postdoctoral fellow with M.J. O’Donohue, TBI Toulouse, France (2006-2007, 12 months); postdoctoral fellow with R. Roy, UQAM, Canada (2005-2006, 20 months)
  • Contribution to research projects (European, national and regional grants): 22, including 12 as principal investigator
  • Co-chair of the international conference “13th Carbohydrate Bioengineering Meeting” (CBM13; Toulouse, France, 2019; 216 attendees from 29 countries)
  • Member of the consortium Catalysis in Toulouse (C@T, 2018-onward)
  • Organizing board member of the “3rd TransPyrenean Meeting in Catalysis” (TraPCat3; Toulouse, France, planned in 2023)
  • Advisor of Toulouse INSA-UPS iGEM’s teams (2017, 2018 & 2020)
  • Reviewer of 6 PhD defenses and for journals: ACS Catal., Angew. Chem. Int. Ed., Biotechnol. Bioeng., Glycobiology, ChemCatChem, Crit. Rev. Biotechnol., etc

List of selected publications

Total of 39 publications

National and international meetings, seminars: 10 as invited speaker as well as 25 oral presentations, 6 poster pitchs, and 45 posters

 

▪ Synthesis of α-l-Araf and β-d-Galf series furanobiosides using mutants of a GH51 α-l-arabinofuranosidase (2021) J. Zhao, J. Esque, I. André, M.J. O’Donohue*, R. FauréBioorg. Chem. 116:105245. DOI: 10.1016/j.bioorg.2021.105245

▪ A tripartite carbohydrate-binding module to functionalize cellulose nanocrystal (2021) A. Pelus, G. Bordes, S. Barbe, Y. Bouchiba, C. Burnard, J. Cortes, B. Enjalbert, J. Esque, A. Estaña, R. Fauré, A. Henras, S. Heux, C. Lemen, P. Millard, S. Nouaille, J. Pérochon, M. Toanen, G. Truan, A. Verdier, C. Wagner, Y. Romeo, C. Montanier  Biomater. Sci. 9:7444-7455. DOI: 10.1039/D1BM01156A

▪ Polysaccharide Utilization Loci-driven enzyme discovery reveals BD-FAE – a bifunctional feruloyl and acetyl xylan esterase active on complex natural xylan from corn fiber (2021) L. Hameleers, L. Penttinen, M. Ikonen, L. Jaillot, R. Fauré, N. Terrapon, N. Hakulinen, E.R. Master, E. Jurak  Biotechnol. Biofuels 14:127. DOI: 10.1186/s13068-021-01976-0

▪ Rational enzyme design without structural knowledge: a sequence-based approach to generate efficient transglycosylases (2021) D. Teze, J. Zhao, M. Wiemann, K.Z.G. Ara, R. Lupo, B. Zeuner, M. Vuillemin, M.E. Rønne, G. Carlström, J.Ø. Duus, Y.-H. Sanejouand, M.J. O’Donohue, E. Nordberg Karlsson, R. Fauré, H. Stålbrand, B. Svensson  Chem. Eur. J. 27:10323-10334. DOI: 10.1002/chem.202100110

▪ Enantioselective reductive oligomerization of carbon dioxide into l-erythrulose via a chemo-enzymatic catalysis (2021) S. Desmons, K. Grayson-Steel, N. Nuñez-Dallos, L. Vendier, J. Hurtado, P. Clapés, R. Fauré, C. Dumon, S. Bontemps  J. Am. Chem. Soc. 143:16274-16283. DOI: 10.1021/jacs.1c07872

▪ Probing the determinants of the transglycosylation/hydrolysis partition in a retaining α-l-arabinofuranosidase (2021) J. Zhao, T. Tandrup, B. Bissaro, S. Barbe, J.-C.N. Poulsen, I. André, C. Dumon, L. Lo Leggio, M.J. O’Donohue*, R. FauréNew Biotechnol. 62:68-78. DOI: 10.1016/j.nbt.2021.01.008

▪ Harnessing glycoenzyme engineering for synthesis of bioactive oligosaccharides (2019) M. Benkoulouche, R. Fauré, M. Remaud-Siméon, C. Moulis, I. André  Interface Focus 9(2):20180069. DOI: 10.1098/rsfs.2018.0069

▪ Formaldehyde as a promising C1 source: the instrumental role of biocatalysis for stereocontrolled reactions (2019) S. Desmons, R. Fauré*, S. Bontemps*  ACS Catal. 9(10):9575-9588. DOI: 10.1021/acscatal.9b03128

▪ A single point mutation alters the transglycosylation/hydrolysis partition, significantly enhancing the synthetic capability of an endo-glycoceramidase (2016) J. Durand, X. Biarnés, L. Watterlot, C. Bonzom, V. Borsenberger, A. Planas, S. Bozonnet, M.J. O’Donohue, R. FauréACS Catal. 6(12):8264-8275. DOI: 10.1021/acscatal.6b02159

▪ Molecular design of non-Leloir furanose-transferring enzymes from an α-l-arabinofuranosidase: a rationale for the engineering of evolved transglycosylases (2015) B. Bissaro, J. Durand, X. Biarnés, A. Planas, P. Monsan, M.J. O’Donohue, R. FauréACS Catal. 5(8):4598-4611. DOI: 10.1021/acscatal.5b00949

▪ Glycosynthesis in a waterworld: new insight into the molecular basis of transglycosylation in retaining glycoside hydrolases (2015) B. Bissaro, P. Monsan, R. Fauré*, M.J. O’Donohue*  Biochem. J. 467(1):17-35. DOI: 10.1042/BJ20141412

▪ A 1H-NMR study of the specificity of α-l-arabinofuranosidases on natural and unnatural substrates (2014) V. Borsenberger, E. Dornez, M.-L. Desrousseaux, S. Massou, M. Tenkanen, C.M. Courtin, C. Dumon, M.J. O’Donohue, R. FauréBBA – Gen. Subjects 1840(10):3106-3114. DOI: 10.1016/j.bbagen.2014.07.001

 

To see all my publications, please check my ORCID profile (0000-0002-5107-9009).