Topic "Adaptive evolution of bacteria under metabolic constraints"

To improve the performance of new microbial cell factories whose metabolism has been rationally modified, a methodology of adaptive evolution under metabolic constraints is applied. The genetic mutations responsible for the observed evolutionary phenomenon are then identified and characterized, in order to propose an adaptive mechanism and to evaluate the robustness and flexibility of bacterial metabolic networks.

This methodology of in vivo adaptive evolution is applied, for example, for the development of a synthetic non-oxidative glycolytic (NOG) pathway functional in anaerobiosis (SynBiochem project) or hydrogen (H₂) production (Hydrogenase project).