Gianluca CIOCI

Actual position and research subject

As research engineer of the National Institute for Agronomic Research (INRAe), I provide my colleagues at TBI my expertise in various fields of structural biology and biophysics such as protein production and purification, X-ray crystallography, cryo-EM, scattering techniques (SAXS, DLS) as well as biophysical techniques (ITC, DSF, CD) and basic molecular modelling. My main research interest is the study of enzyme molecular mechanisms through the combination of multidisciplinary approaches. I actively work on the structure-activity relationships of various classes of enzymes such as glucansucrases, glycosyltransferases, glycosyl hydrolases, phosphorylases and depolymerases. Within the PICT-ICEO platform, I am the responsible of the protein purification & characterization facility, teaching students and helping researchers on how to produce, purify and characterize proteins.

Education and Diploma

Teaching

  • “Protein crystallography and cryo-electron microscopy”, Enzyme Systems module, Master Industrial BioTechnology for a Bio-Based Economy (BioTechEco), INSA Toulouse, France.
  • “Practical Protein Purification”, Continuing Education Unit, INSA Toulouse, France.

Others

  • Co-supervised: 3 PhD students
  • Contribution to research projects:
    • ANR – SPPECABBE, BAMBI, PENTOVAL, REFLEX, ProBio3, CarbUnivax, DECO

    • FUI – Biocatarom

    • ADEME – BioImpulse

    • ERA-IB – BioSurf

    • FP7 –  BABETHANOL, BIOCORE, OPTIBIOCAT

List of selected publications

  • Molina M, Cioci G, Moulis C, Séverac E, Remaud-Siméon M. “Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure-Function Relationship Studies and Engineering” Microorganisms. 2021 Jul 28;9(8):1607. doi: 10.3390/microorganisms9081607.

 

  • Molina M, Moulis C, Monties N, Guieysse D, Morel S, Cioci G,* Remaud-Siméon M.* “A specific oligosaccharide-binding site in the alternansucrase catalytic domain mediates alternan elongation.” J. Biol. Chem. 2020 Jul 10;295(28):9474-9489. doi: 10.1074/jbc.RA120.013028.

 

  • Herviou P, Le Bras M, Dumas L, Hieblot C, Gilhodes J, Cioci G, Hugnot JP,Ameadan A, Guillonneau F, Dassi E, Cammas A, Millevoi S.* « hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma” Nat Commun. 2020 May 27;11(1):2661. doi: 10.1038/s41467-020-16168-x.

 

  • Tournier V, Topham CM, Gilles A, David B, Folgoas C, Moya-Leclair E, Kamionka E, Desrousseaux ML, Texier H, Gavalda S, Cot M, Guémard E, Dalibey M, Nomme J, Cioci G, Barbe S, Chateau M, André I,* Duquesne S,* Marty A.* “An engineered PET depolymerase to break down and recycle plastic bottles”. Nature 2020 Apr;580(7802):216-219. doi: 10.1038/s41586-020-2149-4.

 

  • Claverie M, Cioci G, Vuillemin M, Bondy P, Remaud-Simeon M, Moulis C.* “Processivity of dextransucrases synthesizing very-high-molar-mass dextran is mediated by sugar-binding pockets in domain V” J. Biol. Chem. 2020 Apr 24;295(17):5602-5613. doi: 10.1074/jbc.RA119.011995.

 

  • Claverie M, Cioci G, Guionnet M, Schörghuber J, Lichtenecker R, Moulis C, Remaud-Simeon M, Lippens G.* “Futile Encounter Engineering of the DSR-M Dextransucrase Modifies the Resulting Polymer Length”. Biochemistry 2019 Jun 25;58(25):2853-2859. doi: 10.1021/acs.biochem.9b00373.

 

  • Molina M, Moulis C, Monties N, Pizzut-Serin S, Guieysse D, Morel S, Cioci G,*Remaud-Simeon M*. “Deciphering an Undecided Enzyme: Investigations of the Structural Determinants Involved in the Linkage Specificity of Alternansucrase” ACS Catal. 2019, 9, 3, 2222–2237https://doi.org/10.1021/acscatal.8b04510.

 

  • Lomthong T, Guicherd M, Cioci G, Duquesne S, Marty A, Lumyong S, Kitpreechavanich V*. “Poly (L-lactide)-Degrading Enzyme from Laceyella sacchari LP175: Cloning, Sequencing, Expression, Characterization and Its Hydrolysis of Poly (L-lactide) Polymer » Chiang Mai Journal of Science 2019, 46 (3), 417-430.