Mickaël CASTELAIN

Mickaël CASTELAIN

Mickaël CASTELAIN

INRAE Research

Equipe TIM

Actual position and research subject

My activities are principally dedicated to (i) adhesion of microorganisms to surfaces and/or to other microorganisms (flocculation, agreggation, biofilms) in order to understand biofouling (cleaning-in-place, immersed sensors) and surface proteins (adhesins, pili, cell wall) and (ii) identification of physical limitations of coupled transfers during biological reactions in a bioeconomy context.

As a physicist, I developped tools to scrutinize adhesion capacity of species (Gram+ and Gram- bacteria, fungi, spores) by quantifying significant parameters (shear-stress flow chamber, optical tweezers, AFM) such mainly as detachment forces, single-molecule force spectra, multiplexed microfluidic combined with OT to understand heterogenity encountered in bioreactors.

I am currently the head of Tranfer-Interface-Mixing group at TBI.

Education and Diploma

List of selected publications

  • Surface Conditioning Effects on Submerged Optical Sensors : A Comparative Study of Fused Silica, Titanium Dioxide, Aluminum Oxide, and Parylene C
    Zibin Nan, Pascal Floquet, Didier Combes, Claire Tendero, Mickaël Castelain.
    Sensors, 2023, 23 (23), pp.9546, 〈10.3390/S23239546〉.
    https://cnrs.hal.science/hal-04350817
  • Pili and other surface proteins influence the structure and the nano-mechanical properties of Lactococcus lactis biofilms.
    Ibrahima Dramé, Christine Lafforgue, Cécile Formosa-Dague, Marie-Pierre Chapot-Chartier, Jean-Christophe Piard, Mickaël Castelain et Etienne Dague
    Scientific Reports, 2021, 11 (1), 〈10.1038/s41598-021-84030-1〉.
    https://hal.laas.fr/hal-03163203
  • Development of Polythiourethane/ ZnO-Based Anti-Fouling Materials and Evaluation of the Adhesion of Staphylococcus aureus and Candida glabrata Using Single-Cell Force Spectroscopy.
    Sophie Klemm, Martina Baum, Haoyi Qiu, Zibin Nan, Mafalda Cavalheiro, Miguel Cacho Teixeira, Claire Tendero, Anna Gapeeva, Rainer Adelung, Etienne Dague, Mickaël Castelain et Cécile Formosa-Dague.
    Nanomaterials 2021, 11, 271. //doi.org/10.3390/nano 11020271
    https://hal.inrae.fr/hal-03136104v1
  • Analysis of Homotypic Interactions of Lactococcus lactis Pili Using Single-Cell Force Spectroscopy.
    Ibrahima Dramé, Cécile Formosa-Dague, Marie-Pierre Chapot-Chartier, Jean-Christophe Piard, Mickaël Castelain, et Etienne Dague
    ACS Applied Materials and Interfaces, 12(19) :21411– 21423, May 2020. doi :
    10.1021/acsami.0c03069.
    https://hal.inrae.fr/hal-02860018
  • The nanomechanical properties of Lactococcus lactis pili are conditioned by the polymerized backbone pilin,
    Mickaël Castelain, Marie-Pierre Duviau, Alexis Canette, Philippe Schmitz, Pascal Loubière, Muriel Cocaign Bousquet, Jean-Christophe Piard et Muriel Mercier Bonin,
    PLoS ONE, 11(3) :np, 2016a. doi : 10.1371/journal.pone.0152053.
    https://hal.archives-ouvertes.fr/hal-01886384
  • Oligomerized backbone pilin helps piliated Lactococcus lactis to withstand shear flow,
    Mickaël Castelain, Marie-Pierre Duviau, Virginie Oxaran, Philippe Schmitz, Muriel Bousquet, Pascal Loubière, Jean-Christophe Piard et Muriel Mercier-Bonin,
    Biofouling, 32(8) :911–923, 2016b. doi : 10.1080/08927014.2016.1213817.
    https://hal.archives-ouvertes.fr/hal-01533909
  • Unraveling the role of surface mucus-binding protein and pili in mucoadhesion of Lactococcus lactis,
    Doan Le, Thi-Ly Tran, Marie-Pierre Duviau, Mickael Meyrand, Yann Guerardel, Mickaël Castelain, Pascal Loubière, Marie-Pierre Chapot-Chartier, Etienne Dague et Muriel Mercier-Bonin,
    PLoS ONE, 8(11) :np, 2013. doi : 10.1371/journal.pone.0079850
    https://hal.archives-ouvertes.fr/hal-01204286
  • Single-cell adhesion probed in-situ using optical tweezers : A case study
    with Saccharomyces cerevisiae,
    Mickaël Castelain, Paul G. Rouxhet, Frédéric Pignon, Albert Magnin, and Jean-Michel Piau,
    Journal of Applied Physics, 111(11), 2012. doi : 10.1063/1.4723566.
    https://hal.inrae.fr/hal-02652364
  • Fast uncoiling kinetics of F1C pili expressed by uropathogenic Escherichia coli are revealed on a single pilus level using force-measuring optical
    tweezers
    Mickaël Castelain, Sarah Ehlers, Jeanna Klinth, Stina Lindberg, Magnus Andersson, Bernt Eric Uhlin, and Ove Axner.
    European Biophysics Journal, 40(3) :305–316, 2011. doi : 10.1007/s00249-010-0648-1
    https://hal.inrae.fr/hal-02644269
  • Unfolding and refolding properties of S pili on extraintestinal pathogenic Escherichia coli,
    Mickaël Castelain, Annita E. Sjöstrom, Erik Fällman, Bernt Eric Uhlin, and Magnus Andersson
    European Biophysics Journal, 39(8) :1105–1115, 2010. doi : 10.1007/s00249-009-0552-8.
    https://hal.inrae.fr/hal-02659055