{"id":13595,"date":"2023-10-26T09:11:46","date_gmt":"2023-10-26T07:11:46","guid":{"rendered":"https:\/\/www.toulouse-biotechnology-institute.fr\/?p=13595"},"modified":"2023-10-26T09:16:10","modified_gmt":"2023-10-26T07:16:10","slug":"un-nouveau-capteur-naturel-du-potentiel-antioxydant-des-molecules","status":"publish","type":"post","link":"https:\/\/www.toulouse-biotechnology-institute.fr\/en\/un-nouveau-capteur-naturel-du-potentiel-antioxydant-des-molecules\/","title":{"rendered":"A new natural sensor for the antioxidant potential of molecules"},"content":{"rendered":"
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In order to monitor and quantify antioxidant production, a team of scientists from the Toulouse biotechnology institute (TBI - INRAE \/ INSA Toulouse \/ CNRS) have just developed a biological quality sensor (biosensor) for the antioxidant potential of molecules, using yeast.<\/strong><\/p>\n

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Article published on October 23, 2023\u00a0<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Antioxidants, protectors of our cells<\/strong><\/h2>\n

Essential supplements and preservatives for human and animal nutrition, antioxidants play a key role in health and nutrition.<\/p>\n

A molecule is said to be antioxidant when it slows down or prevents the oxidation of other chemical substances. Oxidation is a classic chemical reaction, notably occurring in cells during the cellular respiration process, where sugar is broken down in the presence of oxygen to release energy. However, these oxidation reactions can lead to the production of free radicals, highly reactive chemical entities that degrade cellular constituents and structures.<\/p>\n

Antioxidants are molecules naturally present in our diet and capable of protecting our cells against these free radicals. At moderate levels, they have beneficial effects. On the other hand, if their concentration becomes too high, they can damage cells, lipids, proteins and DNA, which can lead to the onset of disease and cancer.<\/p>\n

Because of their protective power, antioxidants are increasingly popular and used in the fields of health, nutrition and well-being. Although a large number of synthetic antioxidants are available, the demand for antioxidants of natural origin is very high. Their bioproduction by microorganisms is more ecological and sustainable, but also highly competitive, and it is important to be able to monitor and quantify it.<\/p>\n

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Yeast as a biosensor of the antioxidant power of molecules<\/strong><\/h2>\n

Scientific studies have already developed ways of controlling and monitoring the synthesis of antioxidant molecules, but some can be restrictive, costly or tricky to implement.1,2 <\/sup>.<\/p>\n

That's why the TBI team wanted to work on a well-known yeast, the brewer's yeast known as Saccharomyces cerevisiae<\/em>.It is one of the best genetically developed eukaryotic (i.e. with a nucleus in the cell) models, with simple nutritional requirements, and is quick and easy to grow.<\/p>\n

Thanks to these yeasts, scientists have developed a simple test enabling the detection and quantification of certain antioxidant molecules such as resveratrol, epigallocatechin gallate, quercetin and astaxanthin, when placed in the presence of yeast. It's a conclusive test for detecting and quantifying antioxidants.<\/p>\n

But the test goes further, as it also shows that it is possible to monitor antioxidant biosynthesis directly in the microorganisms that produce them. By genetically modifying a yeast plant, scientists have monitored and quantified the synthesis of a \"natural\" type of antioxidant: carotenoids.<\/p>\n

In addition to its ease of implementation, this yeast-based quantification method also makes it possible to measure, characterize and study the physiological effect of antioxidant molecules bioproduced by microorganisms. It could therefore be very useful for improving the strains of microorganisms commonly used for antioxidant production.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\n\t\t\t\t\t\tGosselin-Monplaisir, T.; Dagkesamanskaya, A.; Rigal, M.; Floch, A.; Furger, C.; Martin-Yken, H. A New Role for Yeast Cells in Health and Nutrition: Antioxidant Power Assessment. Int. J. Mol. Sci. 2023, 24, 11800. https:\/\/doi.org\/10.3390\/ijms241411800\n1Wolfe, K.L.; Liu, R.H. Cellular Antioxidant Activity (CAA) assay for assessing antioxidants, foods, and dietary supplements. J. Agric. Food Chem. 2007, 55, 8896\u20138907. \n2Gironde, C.; Rigal, M.; Dufour, C.; Furger, C. AOP1, a new live cell assay for the direct and quantitative measure of intracellular antioxidant effects. Antioxidants 2020, 9, 471\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\tH\u00e9l\u00e8ne MARTIN-YKEN, Charg\u00e9e de recherche INRAE
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Afin de pouvoir suivre et quantifier la production d\u2019antioxydant, une \u00e9quipe de scientifiques du laboratoire Toulouse biotechnology institute (TBI – INRAE \/ INSA Toulouse \/ CNRS) vient de mettre au point un capteur de qualit\u00e9 biologique (biosenseur) du potentiel antioxydant des mol\u00e9cules en utilisant une levure. \u00a0 Article publi\u00e9 le 23 octobre 2023\u00a0 A lire […]<\/p>\n","protected":false},"author":1,"featured_media":13596,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[12],"tags":[36],"class_list":["post-13595","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-resultat-scientifique","tag-equipe-i2m"],"yoast_head":"\nUn nouveau capteur naturel du potentiel antioxydant des mol\u00e9cules | TBI, Toulouse Biotechnology Institute<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, 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