La soutenance aura lieu \u00e0 9h15 en salle 401 (bat. 39) le vendredi 29 septembre<\/p>\n\n\n\n
Title: <\/strong>Beyond ABE fermentation: metabolic engineering of Clostridium acetobutylicum<\/em> for the production of alternative metabolites<\/p>\n\n\n\n Abstract<\/strong><\/p>\n\n\n\n The strict anaerobe Clostridium acetobutylicum<\/em> has a century-long history with industrial biotechnology due to its ability to produce acetone and butanol from a wide range of carbohydrates. Our team has recently demonstrated that C. acetobutylicum can also efficiently degrade lignocellulosic substrates thanks to specific genome editions, making it a promising candidate for consolidated bioprocessing. In this context, my thesis describes metabolic engineering strategies to obtain C. acetobutylicum<\/em> mutants producing fermentation products of industrial relevance.<\/p>\n\n\n\n A CRISPR\/Cas9 tool was used to delete the genes ptb-buk, ldhA <\/em>and thlA <\/em>which resulted in a C. acetobutylicum mutant encoding the remaining key elements for NAD(P)H oxidation on the genomic megaplasmid pSOL1. This platform strain was then further used to insert and optimise the NADH oxidation pathways of compounds that C. acetobutylicum<\/em> normally only produces in small amounts. In particular, we investigated the production of lactate, glycerol, 1,3-propanediol, ethanol and specific amino acids.<\/p>\n\n\n\n Jury:<\/strong><\/p>\n\n\n\n M. Luis Ba\u00f1eras Vives, Universitat de Girona, Rapporteur<\/p>\n\n\n\n M. Henri-Pierre Fierobe, CNRS, Rapporteur<\/p>\n\n\n\n Mme Isabelle Meynial-Salles, INSA Toulouse, Examinatrice<\/p>\n\n\n\n M. Wolfgang Liebl, Technische Universit\u00e4t M\u00fcnchen, Examinateur<\/p>\n\n\n\n M. Philippe Soucaille, INSA Toulouse, Directeur de th\u00e8se<\/p>\n\n\n\n M. Tom Wilding-Steele, INSA Toulouse, Co-encadrant de th\u00e8se<\/p>\n<\/blockquote>","protected":false},"excerpt":{"rendered":" September 29, 2023<\/p>","protected":false},"author":1,"featured_media":13317,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[10,75],"tags":[69],"class_list":["post-13316","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evenement","category-soutenance","tag-equipe-peep"],"yoast_head":"\n\n