{"id":11781,"date":"2023-03-02T09:54:11","date_gmt":"2023-03-02T08:54:11","guid":{"rendered":"https:\/\/www.toulouse-biotechnology-institute.fr\/?p=11781"},"modified":"2023-04-06T16:42:06","modified_gmt":"2023-04-06T14:42:06","slug":"nouvelles-technologies-microfluidiques-pour-accelerer-la-decouverte-et-lingenierie-denzymes-voies-metaboliques-souches-et-consortia-microbiens","status":"publish","type":"post","link":"https:\/\/www.toulouse-biotechnology-institute.fr\/en\/nouvelles-technologies-microfluidiques-pour-accelerer-la-decouverte-et-lingenierie-denzymes-voies-metaboliques-souches-et-consortia-microbiens\/","title":{"rendered":"Novel microfluidic technologies to accelerate the discovery and engineering of microbial enzymes, metabolic pathways, strains and consortia"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"11781\" class=\"elementor elementor-11781\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-07a2bf1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"07a2bf1\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-629046a\" data-id=\"629046a\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b11131a elementor-widget elementor-widget-text-editor\" data-id=\"b11131a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p><strong>Les \u00e9cosyst\u00e8mes microbiens repr\u00e9sentent une mine d\u2019or pour la prospection de nouvelles fonctions d\u2019int\u00e9r\u00eat biotechnologique. Pourtant, la majorit\u00e9 des esp\u00e8ces qui composent ces communaut\u00e9s complexes ne sont pas cultiv\u00e9es et constituent une bo\u00eete noire dont le fonctionnement est difficile, voire impossible \u00e0 \u00e9tudier. Pour faire face \u00e0 ces enjeux, le TBI et TWB , en collaboration avec l\u2019unit\u00e9 INSERM I2MC, ont int\u00e9gr\u00e9 microbiomics et microfluidique en gouttes pour d\u00e9velopper une nouvelle strat\u00e9gie de criblage \u00e0 ultra-haut d\u00e9bit pour la culturomique, la m\u00e9tagenomique fonctionnelle, l\u2019ing\u00e9nierie d\u2019enzymes, de souches et de consortia microbiens. Deux technologies de rupture, qui ne reposent ni sur l&rsquo;utilisation de substrats fluorog\u00e9niques ni sur des banques fluorescentes, ont \u00e9t\u00e9 d\u00e9velopp\u00e9es et brevet\u00e9es par l\u2019INRAE. Elles sont actuellement exploit\u00e9es dans le cadre du projet ANR CAZIBD pour d\u00e9crypter le dialogue h\u00f4te-microbiote-aliment, et du projet europ\u00e9en BLUETOOLS d\u00e9di\u00e9 \u00e0 l\u2019\u00e9tude et l\u2019exploitation biotechnologique des \u00e9cosyst\u00e8mes marins.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6320a4c elementor-widget elementor-widget-text-editor\" data-id=\"6320a4c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p>Microfluidics allows coupling ultra-high throughput isolation and culture of microorganisms in drops with phenotypic, taxonomic and genomic characterization. However, existing technologies require either labeled recombinant cell banks, cell survival markers, or fluorogenic or chromogenic probes or substrates. They are therefore not adapted to the culture and screening of native microorganisms, nor to the use of non-chemically modified substrates naturally present in microbial ecosystems. These barriers limit the understanding of the functioning of ecosystems to their biotechnological exploitation.<\/p><p>\u00a0<\/p><p class=\"translation-block\">Two revolutionary technologies, based on different principles, have been developed. The first integrates drop microfluidics, imaging, artificial intelligence and flow cytometry for automated detection and sorting of positive drops, at a rate of about 10<sup>5<\/sup> drops per hour. The second technology, dedicated to culturomics and strain and consortia engineering, directly couples drop microfluidics and flow cytometry for the selection of growing microorganisms, at a rate of 10<sup>6<\/sup> drops per hour. With a picoliter scale, a million times smaller than the sample volumes used with conventional phenotypic screening techniques, these technologies are compatible with any type of substrate at any cost, and the screening of any phenotype, as long as it can be detected by confocal microscopy or linked to microbial cell growth.<\/p><p>\u00a0<\/p><p>Les technologies d\u00e9velopp\u00e9es peuvent \u00eatre exploit\u00e9es pour la m\u00e9tag\u00e9nomique fonctionnelle, la culturomique, l&rsquo;ing\u00e9nierie d\u2019enzymes m\u00eame en utilisant des syst\u00e8mes acellulaires, l\u2019ing\u00e9nierie de souches ou de consortia, par exemple pour la production d\u2019antimicrobiens ou pour la d\u00e9gradation de polym\u00e8res synth\u00e9tiques polluants tels que les plastiques.<\/p><p>\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c420883 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"c420883\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d15b3ba elementor-position-inline-start elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"d15b3ba\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"icon-box.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-far-star\" viewbox=\"0 0 576 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M528.1 171.5L382 150.2 316.7 17.8c-11.7-23.6-45.6-23.9-57.4 0L194 150.2 47.9 171.5c-26.2 3.8-36.7 36.1-17.7 54.6l105.7 103-25 145.5c-4.5 26.3 23.2 46 46.4 33.7L288 439.6l130.7 68.7c23.2 12.2 50.9-7.4 46.4-33.7l-25-145.5 105.7-103c19-18.5 8.5-50.8-17.7-54.6zM388.6 312.3l23.7 138.4L288 385.4l-124.3 65.3 23.7-138.4-100.6-98 139-20.2 62.2-126 62.2 126 139 20.2-100.6 98z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tValorization\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tExploitation of these technologies in the framework of the ANR CAZIBD (Mucus-degrading enzymes and their role in intestinal inflammation) and the HORIZON BLUETOOLS project (Innovative tools for sustainable exploration of marine microbiominnovative tools for sustainable exploration of marine microbiomes : towards a circular blue bioeconomy and healthier marine environments)\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-583ff29 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"583ff29\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-efb8927 elementor-position-inline-start elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"efb8927\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"icon-box.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fas-tag\" viewbox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M0 252.118V48C0 21.49 21.49 0 48 0h204.118a48 48 0 0 1 33.941 14.059l211.882 211.882c18.745 18.745 18.745 49.137 0 67.882L293.823 497.941c-18.745 18.745-49.137 18.745-67.882 0L14.059 286.059A48 48 0 0 1 0 252.118zM112 64c-26.51 0-48 21.49-48 48s21.49 48 48 48 48-21.49 48-48-21.49-48-48-48z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tPatents\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\t- S. Lajus, Flores-Flores R., Lestrade D., Deroite A., Dagkesamanskaya, Potocki-Veronese G. A\nnew droplet micro-to-milli-fluidics-based process to screen for phenotypes or biological processes.\nBrevet europ\u00e9en d\u00e9pos\u00e9 par INRAE le 20\/05\/2022 sous le N\u00b0EP22305758.9 <br>\n- S. Lajus, Lestrade D., Deroite A., Dagkesamanskaya, Potocki-Veronese G. New milli-to-microfluidics-based process to screen microbial growth in droplets. Brevet europ\u00e9en d\u00e9pos\u00e9 par INRAE le 20\/05\/2022 sous le N\u00b0 EP22305759.7\n\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a8d3a54 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"a8d3a54\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9af225e elementor-position-inline-start elementor-view-default elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"9af225e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"icon-box.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span  class=\"elementor-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fas-street-view\" viewbox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M367.9 329.76c-4.62 5.3-9.78 10.1-15.9 13.65v22.94c66.52 9.34 112 28.05 112 49.65 0 30.93-93.12 56-208 56S48 446.93 48 416c0-21.6 45.48-40.3 112-49.65v-22.94c-6.12-3.55-11.28-8.35-15.9-13.65C58.87 345.34 0 378.05 0 416c0 53.02 114.62 96 256 96s256-42.98 256-96c0-37.95-58.87-70.66-144.1-86.24zM256 128c35.35 0 64-28.65 64-64S291.35 0 256 0s-64 28.65-64 64 28.65 64 64 64zm-64 192v96c0 17.67 14.33 32 32 32h64c17.67 0 32-14.33 32-32v-96c17.67 0 32-14.33 32-32v-96c0-26.51-21.49-48-48-48h-11.8c-11.07 5.03-23.26 8-36.2 8s-25.13-2.97-36.2-8H208c-26.51 0-48 21.49-48 48v96c0 17.67 14.33 32 32 32z\"><\/path><\/svg>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\n\t\t\t\t\t\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t\t<span  >\n\t\t\t\t\t\t\tContacts\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<p class=\"elementor-icon-box-description\">\n\t\t\t\t\t\tGabrielle Potocki-Veronese (veronese@insa-toulouse.fr) <br>\nSophie Lajus (lajus@insa-toulouse.fr)<br>\nDelphine Lestrade (delphine.lestrade@inrae.fr)\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7e119d8 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"7e119d8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7116159 elementor-widget elementor-widget-text-editor\" data-id=\"7116159\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p><strong>L\u00e9gende et copyright image : <\/strong>Apprentissage pour la d\u00e9tection automatis\u00e9e et le tri de gouttes pour le criblage \u00e0 ultra-haut d\u00e9bit de fonctions microbiennes (Sophie Lajus et Remy Flores-Flores)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Les \u00e9cosyst\u00e8mes microbiens repr\u00e9sentent une mine d\u2019or pour la prospection de nouvelles fonctions d\u2019int\u00e9r\u00eat biotechnologique. Pourtant, la majorit\u00e9 des esp\u00e8ces qui composent ces communaut\u00e9s complexes ne sont pas cultiv\u00e9es et constituent une bo\u00eete noire dont le fonctionnement est difficile, voire impossible \u00e0 \u00e9tudier. Pour faire face \u00e0 ces enjeux, le TBI et TWB , en [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":12078,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[12],"tags":[35],"class_list":["post-11781","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-resultat-scientifique","tag-equipe-cimes"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Nouvelles technologies microfluidiques pour acc\u00e9lerer la d\u00e9couverte et l\u2019ing\u00e9nierie d\u2019enzymes, voies m\u00e9taboliques, souches et consortia microbiens | TBI, Toulouse Biotechnology Institute<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.toulouse-biotechnology-institute.fr\/en\/nouvelles-technologies-microfluidiques-pour-accelerer-la-decouverte-et-lingenierie-denzymes-voies-metaboliques-souches-et-consortia-microbiens\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Nouvelles technologies microfluidiques pour acc\u00e9lerer la d\u00e9couverte et l\u2019ing\u00e9nierie d\u2019enzymes, voies m\u00e9taboliques, souches et consortia microbiens | TBI, Toulouse Biotechnology Institute\" \/>\n<meta property=\"og:description\" content=\"Les \u00e9cosyst\u00e8mes microbiens repr\u00e9sentent une mine d\u2019or pour la prospection de nouvelles fonctions d\u2019int\u00e9r\u00eat biotechnologique. Pourtant, la majorit\u00e9 des esp\u00e8ces qui composent ces communaut\u00e9s complexes ne sont pas cultiv\u00e9es et constituent une bo\u00eete noire dont le fonctionnement est difficile, voire impossible \u00e0 \u00e9tudier. Pour faire face \u00e0 ces enjeux, le TBI et TWB , en [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.toulouse-biotechnology-institute.fr\/en\/nouvelles-technologies-microfluidiques-pour-accelerer-la-decouverte-et-lingenierie-denzymes-voies-metaboliques-souches-et-consortia-microbiens\/\" \/>\n<meta property=\"og:site_name\" content=\"TBI, Toulouse Biotechnology Institute\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/TBI-Toulouse-Biotechnology-Institute-Bio-Chemical-Engineering-360428497952306\" \/>\n<meta property=\"article:published_time\" content=\"2023-03-02T08:54:11+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-04-06T14:42:06+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.toulouse-biotechnology-institute.fr\/wp-content\/uploads\/2023\/03\/microfluidiques.png\" \/>\n\t<meta property=\"og:image:width\" content=\"454\" \/>\n\t<meta property=\"og:image:height\" content=\"252\" \/>\n\t<meta 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class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.toulouse-biotechnology-institute.fr\\\/nouvelles-technologies-microfluidiques-pour-accelerer-la-decouverte-et-lingenierie-denzymes-voies-metaboliques-souches-et-consortia-microbiens\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.toulouse-biotechnology-institute.fr\\\/nouvelles-technologies-microfluidiques-pour-accelerer-la-decouverte-et-lingenierie-denzymes-voies-metaboliques-souches-et-consortia-microbiens\\\/\"},\"author\":{\"name\":\"Christelle\",\"@id\":\"https:\\\/\\\/www.toulouse-biotechnology-institute.fr\\\/#\\\/schema\\\/person\\\/925c08c7f4d7092aa300f21b872c6dfe\"},\"headline\":\"Nouvelles technologies microfluidiques pour acc\u00e9lerer la d\u00e9couverte et l\u2019ing\u00e9nierie d\u2019enzymes, voies m\u00e9taboliques, souches et consortia 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