{"id":11781,"date":"2023-03-02T09:54:11","date_gmt":"2023-03-02T08:54:11","guid":{"rendered":"https:\/\/www.toulouse-biotechnology-institute.fr\/?p=11781"},"modified":"2023-04-06T16:42:06","modified_gmt":"2023-04-06T14:42:06","slug":"nouvelles-technologies-microfluidiques-pour-accelerer-la-decouverte-et-lingenierie-denzymes-voies-metaboliques-souches-et-consortia-microbiens","status":"publish","type":"post","link":"https:\/\/www.toulouse-biotechnology-institute.fr\/en\/nouvelles-technologies-microfluidiques-pour-accelerer-la-decouverte-et-lingenierie-denzymes-voies-metaboliques-souches-et-consortia-microbiens\/","title":{"rendered":"Novel microfluidic technologies to accelerate the discovery and engineering of microbial enzymes, metabolic pathways, strains and consortia"},"content":{"rendered":"
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Microbial ecosystems represent a gold mine for the exploration of new functions of biotechnological interest. However, most of the species that make up these complex communities are not cultivated and constitute a black box whose functioning is difficult, if not impossible to study. To address these challenges, the TBI and TWB, in collaboration with the INSERM I2MC unit, have integrated microbiomics and microfluidic droplet technology to develop a new ultra-high-throughput screening strategy for culturomics, functional metagenomics, and the engineering of enzymes, strains and microbial consortia. Two breakthrough technologies, which do not rely on fluorogenic substrates or fluorescent libraries, have been developed and patented by INRAE. They are currently used in the framework of the ANR CAZIBD project to decipher the host-microbiota-food dialogue, and the European BLUETOOLS project dedicated to the study and biotechnological exploitation of marine ecosystems.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Microfluidics allows coupling ultra-high throughput isolation and culture of microorganisms in drops with phenotypic, taxonomic and genomic characterization. However, existing technologies require either labeled recombinant cell banks, cell survival markers, or fluorogenic or chromogenic probes or substrates. They are therefore not adapted to the culture and screening of native microorganisms, nor to the use of non-chemically modified substrates naturally present in microbial ecosystems. These barriers limit the understanding of the functioning of ecosystems to their biotechnological exploitation.<\/p>

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Two revolutionary technologies, based on different principles, have been developed. The first integrates drop microfluidics, imaging, artificial intelligence and flow cytometry for automated detection and sorting of positive drops, at a rate of about 105<\/sup> drops per hour. The second technology, dedicated to culturomics and strain and consortia engineering, directly couples drop microfluidics and flow cytometry for the selection of growing microorganisms, at a rate of 106<\/sup> drops per hour. With a picoliter scale, a million times smaller than the sample volumes used with conventional phenotypic screening techniques, these technologies are compatible with any type of substrate at any cost, and the screening of any phenotype, as long as it can be detected by confocal microscopy or linked to microbial cell growth.<\/p>

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The technologies developed can be exploited for functional metagenomics, culturomics, enzyme engineering even using cell-free systems, engineering of strains or consortia, e.g. for the production of antimicrobials or for the degradation of polluting synthetic polymers such as plastics.<\/p>

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\n\t\t\t\t\t\tExploitation of these technologies in the framework of the ANR CAZIBD (Mucus-degrading enzymes and their role in intestinal inflammation) and the HORIZON BLUETOOLS project (Innovative tools for sustainable exploration of marine microbiominnovative tools for sustainable exploration of marine microbiomes : towards a circular blue bioeconomy and healthier marine environments)\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\t- S. Lajus, Flores-Flores R., Lestrade D., Deroite A., Dagkesamanskaya, Potocki-Veronese G. A\nnew droplet micro-to-milli-fluidics-based process to screen for phenotypes or biological processes.\nBrevet europ\u00e9en d\u00e9pos\u00e9 par INRAE le 20\/05\/2022 sous le N\u00b0EP22305758.9
\n- S. Lajus, Lestrade D., Deroite A., Dagkesamanskaya, Potocki-Veronese G. New milli-to-microfluidics-based process to screen microbial growth in droplets. Brevet europ\u00e9en d\u00e9pos\u00e9 par INRAE le 20\/05\/2022 sous le N\u00b0 EP22305759.7\n\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tContacts\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

\n\t\t\t\t\t\tGabrielle Potocki-Veronese (veronese@insa-toulouse.fr)
\nSophie Lajus (lajus@insa-toulouse.fr)
\nDelphine Lestrade (delphine.lestrade@inrae.fr)\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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L\u00e9gende et copyright image : <\/strong>Apprentissage pour la d\u00e9tection automatis\u00e9e et le tri de gouttes pour le criblage \u00e0 ultra-haut d\u00e9bit de fonctions microbiennes (Sophie Lajus et Remy Flores-Flores)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

Les \u00e9cosyst\u00e8mes microbiens repr\u00e9sentent une mine d\u2019or pour la prospection de nouvelles fonctions d\u2019int\u00e9r\u00eat biotechnologique. Pourtant, la majorit\u00e9 des esp\u00e8ces qui composent ces communaut\u00e9s complexes ne sont pas cultiv\u00e9es et constituent une bo\u00eete noire dont le fonctionnement est difficile, voire impossible \u00e0 \u00e9tudier. Pour faire face \u00e0 ces enjeux, le TBI et TWB , en […]<\/p>\n","protected":false},"author":1,"featured_media":12078,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[12],"tags":[35],"class_list":["post-11781","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-resultat-scientifique","tag-equipe-cimes"],"yoast_head":"\nNouvelles technologies microfluidiques pour acc\u00e9lerer la d\u00e9couverte et l\u2019ing\u00e9nierie d\u2019enzymes, voies m\u00e9taboliques, souches et consortia microbiens | TBI, Toulouse Biotechnology Institute<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.toulouse-biotechnology-institute.fr\/en\/nouvelles-technologies-microfluidiques-pour-accelerer-la-decouverte-et-lingenierie-denzymes-voies-metaboliques-souches-et-consortia-microbiens\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Nouvelles technologies microfluidiques pour acc\u00e9lerer la d\u00e9couverte et l\u2019ing\u00e9nierie d\u2019enzymes, voies m\u00e9taboliques, souches et consortia microbiens | TBI, Toulouse Biotechnology Institute\" \/>\n<meta property=\"og:description\" content=\"Les \u00e9cosyst\u00e8mes microbiens repr\u00e9sentent une mine d\u2019or pour la prospection de nouvelles fonctions d\u2019int\u00e9r\u00eat biotechnologique. Pourtant, la majorit\u00e9 des esp\u00e8ces qui composent ces communaut\u00e9s complexes ne sont pas cultiv\u00e9es et constituent une bo\u00eete noire dont le fonctionnement est difficile, voire impossible \u00e0 \u00e9tudier. Pour faire face \u00e0 ces enjeux, le TBI et TWB , en […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.toulouse-biotechnology-institute.fr\/en\/nouvelles-technologies-microfluidiques-pour-accelerer-la-decouverte-et-lingenierie-denzymes-voies-metaboliques-souches-et-consortia-microbiens\/\" \/>\n<meta property=\"og:site_name\" content=\"TBI, Toulouse Biotechnology Institute\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/TBI-Toulouse-Biotechnology-Institute-Bio-Chemical-Engineering-360428497952306\" \/>\n<meta property=\"article:published_time\" content=\"2023-03-02T08:54:11+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-04-06T14:42:06+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.toulouse-biotechnology-institute.fr\/wp-content\/uploads\/2023\/03\/microfluidiques.png\" \/>\n\t<meta property=\"og:image:width\" content=\"454\" \/>\n\t<meta property=\"og:image:height\" content=\"252\" \/>\n\t<meta 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