{"id":10258,"date":"2022-07-06T08:10:18","date_gmt":"2022-07-06T06:10:18","guid":{"rendered":"https:\/\/www.toulouse-biotechnology-institute.fr\/?p=10258"},"modified":"2023-01-25T10:40:56","modified_gmt":"2023-01-25T09:40:56","slug":"soutenance-de-these-fan-chen","status":"publish","type":"post","link":"https:\/\/www.toulouse-biotechnology-institute.fr\/en\/soutenance-de-these-fan-chen\/","title":{"rendered":"Defense of thesis Fan CHEN"},"content":{"rendered":"

PhD defence entitled “ Exploring the role of 5’UTRs in the regulation of gene expression using synthetic and native libraries<\/strong>“, supervised by Laurence Girbal and S\u00e9bastien Nouaille.<\/p>\n\n\n\n

The PhD defense will be on July 6 at 2 pm <\/strong>in visioconference.<\/p>\n\n\n\n

Abstract<\/strong><\/p>\n\n\n\n

When facing changing environments, bacteria have to adapt their metabolism by modifying the expression pattern of their gene repertoire. The goal of my PhD was to provide additional fundamental insights into gene expression regulation in bacteria. We have focused our work on the role of 5\u2019UTRs in gene expression regulation in E. coli<\/em>. 5\u2019UTRs are the transcribed but untranslated sequences located in the 5\u2019ends of the mRNAs.<\/p>\n\n\n\n

We first developed an approach using synthetic 5\u2019UTR sequences to analyze their role at three levels, namely translation, transcription, and mRNA degradation. We confirmed the multilevel contribution of 5\u2019UTRs in the control of gene expression at the level of translation initiation, transcription, and\/or mRNA stability and showed the degree of dependence on the downstream reporter gene sequence. Then we played with the 5\u2019UTR-mediated translation regulation and showed the consequences on mRNA concentration and stability.<\/p>\n\n\n\n

How 5\u2019UTRs regulate gene expression in response to environmental changes is still only partially understood. We designed and constructed a full-size library of 2547 native 5\u2019UTR sequences from E. coli<\/em> fused to a fluorescent reporter gene. The role of native 5\u2019UTRs was explored by challenging the native 5\u2019UTR library to grow in changing environmental conditions. We demonstrated that the 5\u2019UTRs directly and efficiently regulate downstream gene expression and thus contribute to the E. coli<\/em> adaption to changing environments.<\/p>","protected":false},"excerpt":{"rendered":"

July 6, 2022<\/p>","protected":false},"author":1,"featured_media":10264,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[10,75],"tags":[27],"class_list":["post-10258","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evenement","category-soutenance","tag-equipe-blade"],"yoast_head":"\nSoutenance de th\u00e8se Fan CHEN | TBI, Toulouse Biotechnology Institute<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.toulouse-biotechnology-institute.fr\/en\/soutenance-de-these-fan-chen\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Soutenance de th\u00e8se Fan CHEN | TBI, Toulouse Biotechnology Institute\" \/>\n<meta property=\"og:description\" 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