{"id":7987,"date":"2022-04-22T19:41:22","date_gmt":"2022-04-22T17:41:22","guid":{"rendered":"http:\/\/v2tbi.christelle-labruyere.com\/?page_id=7987"},"modified":"2022-06-08T18:24:32","modified_gmt":"2022-06-08T16:24:32","slug":"metabolisme","status":"publish","type":"page","link":"https:\/\/www.toulouse-biotechnology-institute.fr\/en\/poles\/equipe-rmn-2\/metabolisme\/","title":{"rendered":"M\u00e9tabolisme dans les extraits de cellules"},"content":{"rendered":"\t\t
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Equipe RMN<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Th\u00e8me \"M\u00e9tabolisme dans les extraits de cellules\"<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Quelques mots sur le th\u00e8me<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Etudier le m\u00e9tabolisme dans des extraits cellulaires actifs <\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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La vie peut \u00eatre d\u00e9compos\u00e9e en un ensemble d\u00e9fini de r\u00e9actions chimiques qui sont catalys\u00e9es par des enzymes et composent un vaste r\u00e9seau dense et hautement connect\u00e9 appel\u00e9 m\u00e9tabolisme. Nous proposons d’\u00e9tudier le m\u00e9tabolisme dans des extraits cellulaires actifs (CFE), obtenus par la lyse des cellules et la s\u00e9paration ult\u00e9rieure de leurs composants solubles. La r\u00e9sonance magn\u00e9tique nucl\u00e9aire (RMN) est un outil bien adapt\u00e9 et non destructif permettant l’identification et la quantification de petites mol\u00e9cules organiques dans une matrice complexe, \u00e0 la fois \u00e0 un moment donn\u00e9 ou tout au long de r\u00e9actions chimiques en temps r\u00e9el.<\/p>

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Nous avons adapt\u00e9 des m\u00e9thodes de RMN pour surveiller des param\u00e8tres physicochimiques tels que le pH simultan\u00e9ment \u00e0 la concentration de m\u00e9tabolites au cours d’une r\u00e9action chimique, et nous avons d\u00e9crit des s\u00e9quences RMN pour l’identification sp\u00e9cifique de m\u00e9tabolites phosphoryl\u00e9s dans des \u00e9chantillons biologiques complexes. Nous \u00e9tudions l’activit\u00e9 glycolytique et la partition voie des pentoses phosphates\/glycolyse dans l’EFC en fonction des pools de cofacteurs. Nos r\u00e9sultats confirment que les cofacteurs peuvent avoir une forte influence sur le flux glycolytique, faisant \u00e9cho \u00e0 ce qui a \u00e9t\u00e9 observ\u00e9 in vivo, mais sugg\u00e8rent \u00e9galement d’autres hypoth\u00e8ses sur le r\u00f4le des cofacteurs sur le m\u00e9tabolisme central.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Les projets<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Projet ExtractNMR<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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Le projet ExtractNMR vise \u00e0 \u00e9largir cette approche aux EFC d’autres micro-organismes.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Equipe RMN Retour vers l’\u00e9quipe Th\u00e8me “M\u00e9tabolisme dans les extraits de cellules” Quelques mots sur le th\u00e8me Etudier le m\u00e9tabolisme dans des extraits cellulaires actifs La vie peut \u00eatre d\u00e9compos\u00e9e en un ensemble d\u00e9fini de r\u00e9actions chimiques qui sont catalys\u00e9es par des enzymes et composent un vaste r\u00e9seau dense et hautement connect\u00e9 appel\u00e9 m\u00e9tabolisme. Nous […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":7929,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-7987","page","type-page","status-publish","hentry"],"yoast_head":"\nM\u00e9tabolisme dans les extraits de cellules | TBI, Toulouse Biotechnology Institute<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.toulouse-biotechnology-institute.fr\/en\/poles\/equipe-rmn-2\/metabolisme\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"M\u00e9tabolisme dans les extraits de cellules | TBI, Toulouse Biotechnology Institute\" \/>\n<meta property=\"og:description\" content=\"Equipe RMN Retour vers l’\u00e9quipe Th\u00e8me “M\u00e9tabolisme dans les extraits de cellules” Quelques mots sur le th\u00e8me Etudier le m\u00e9tabolisme dans des extraits cellulaires actifs La vie peut \u00eatre d\u00e9compos\u00e9e en un ensemble d\u00e9fini de r\u00e9actions chimiques qui sont catalys\u00e9es par des enzymes et composent un vaste r\u00e9seau dense et hautement connect\u00e9 appel\u00e9 m\u00e9tabolisme. 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