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Régis FAURÉ

 

Régis FAURÉ

CNRS senior research scientist

Enzyme-based Catalysis team

+33 (0) 561 559 410

regis.faure @ insa-toulouse.fr

 

PUBLICATIONS

CV

CNRS senior research scientist (Enzyme Catalysis, Bioorganic Chemistry & Biomolecular Engineering), TBI (ex-LISBP), INSA Toulouse, France (2007-onward)

Biomolecular engineering: design and molecular study of bio-inspired evolved and/or artificial enzymatic catalysts, and glycosylation of proteins

Co-chair of the 13th Carbohydrate Bioengineering Meeting (CBM13, Toulouse, 2019)

Accreditation to supervise research (HDR), UPS Toulouse (2017)

Visiting scientist with A. Planas, IQS Barcelona, Spain (2015)

Postdoctoral fellow with M.J. O’Donohue, LISBP Toulouse, France (2006-2007)

Postdoctoral fellow with R. Roy, UQAM, Canada (2005-2006)

PhD in Biological Chemistry with H. Driguez & S. Cottaz (CERMAV), UJF Grenoble, France (2004)

MSc in Biological Chemistry, UPS, Toulouse, France (2000)

BSc in Physical Chemistry, UPS Toulouse, France (1998)

 

 

Research Topics

Since joining the CNRS as a researcher in the LISBP laboratory (recently renamed TBI), I pursued the development of my skills in organic synthesis, while acquiring new knowledge and practical knowhow in the fields of protein biochemistry, enzyme engineering and high-throughput screening. In my laboratory, I supply vital in-house expertise in the field of (glyco)chemistry, supporting research focused on enzyme discovery through the design of ad hoc substrates for easy specific detection of enzymatic activities. I also provide strong input in the area of enzymatic (glyco)synthesis, using carbohydrate active enzymes (transglycosylases from evolved glycoside hydrolases, esterases, etc.) and work on development of novel enzymes for de novo synthesis of carbohydrates. Most recently, I have shifted a part of my research activity towards the redesign of enzyme active sites, also targeting protein glycosylation as an applied aim. This research calls upon my knowledge of chemistry and uses non-canonical amino acids to extend the chemical functionalities of naturally-occurring enzymes.

 

 

Selected Publications

Rational enzyme design without structural knowledge: a sequence-based approach for efficient generation of glycosylation catalysts (2020) D. Teze, J. Zhao, M. Wiemann, K.Z.G. Ara, R. Lupo, G. Carlström, J. Duus, Y.-H. Sanejouand, M.J. O'Donohue, E. Nordberg-Karlsson, R. Fauré, H. Stålbrand, B. Svensson ChemRxiv

 

A single point mutation alters the transglycosylation/hydrolysis partition, significantly enhancing the synthetic capability of an endo-glycoceramidase. (2016) J. Durand, X. Biarnés, L. Watterlot, C. Bonzom, V. Borsenberger, A. Planas, S. Bozonnet, M.J. O’Donohue, R. Fauré* ACS Catal. 6(12):8264-8275. DOI: 10.1021/acscatal.6b02159

 

Design of chromogenic probes for efficient screening and evaluation of feruloyl esterase-like activities (2016) O. Gherbovet#, R. Fauré#,*, F. Ferreira, J. Durand, M. Ragon, G. Hostyn, E. Record, S. Bozonnet, M.J. O'Donohue* J. Mol. Catal. B: Enzym. 126:24-31. DOI: 10.1016/j.molcatb.2016.01.012

 

Molecular design of non-Leloir furanose-transferring enzymes from an α-L-arabinofuranosidase: a rationale for the engineering of evolved transglycosylases (2015) B. Bissaro, J. Durand, X. Biarnés, A. Planas, P. Monsan, M.J. O'Donohue* and R. Fauré* ACS Catal. 5(8):4598-4611. DOI: 10.1021/acscatal.5b00949

 

Glycosynthesis in a Waterworld: new insight into the molecular basis of transglycosylation in retaining glycoside hydrolases (2015) B. Bissaro, P. Monsan, R. Fauré* and M.J. O'Donohue* Biochem. J. 467(1):17-35. DOI: 10.1042/BJ20141412

 

A 1H-NMR study of the specificity of α-l-arabinofuranosidases on natural and unnatural substrates (2014) V. Borsenberger, E. Dornez, M.-L. Desrousseaux, S. Massou, M. Tenkanen, C.M. Courtin, C. Dumon, M.J. O'Donohue, R. Fauré* BBA - Gen. Subjects 1840(10):3106-3114. DOI: 10.1016/j.bbagen.2014.07.001

 

A brief and informationally rich naming system for oligosaccharide motifs of heteroxylans found in plant cell walls (2009) R. Fauré, C.M. Courtin, J.A. Delcour, C. Dumon, C.B. Faulds, G.B. Fincher, S. Fort, S.C. Fry, S. Halila, M.A. Kabel, L. Pouvreau, B. Quemener, A. Rivet, L. Saulnier, H.A. Schols, H. Driguez, M.J. O’Donohue Aus. J. Chem. 62(6):533-537. DOI: 10.1071/CH08458

 

En route to a carbohydrate-based vaccine against Burkholderia cepacia (2007) R. Fauré, T.C. Shiao, D. Lagnoux, D. Giguère, R. Roy Org. Biomol. Chem. 5(16):2704-2708. DOI: 10.1039/B708365C

 

Synthesis of a library of xylogluco-oligosaccharides for active-site mapping of xyloglucan endo-transglycosylase (2006) R. Fauré, M. Saura-Valls, H. Brumer III, A. Planas, S. Cottaz, H. Driguez J. Org. Chem. 71(14):5151-5161. DOI: 10.1021/jo0525682