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Mathieu Spérandio

Mathieu Spérandio

43 years




INSA de Toulouse

+33 (0) 5 61 55 97 55

135 avenue de Rangueil

31077 Toulouse cedex 4 - FRANCE

sperandio @ insa-toulouse.fr



Professor at the National Institute of Applied Sciences in the Department of

Chemical and Environmental Engineering (from 2008) http://www.insa-toulouse.fr

Head of the team “Microbial Ecosystem and Bioprocesses for wastewater and waste management” (Symbiose) at the laboratory LISBP (from 2009). www.lisbp.fr

Management committee of the IWA Specialist Group on Nutrient removal and recovery from IWA (International Water association) (from 2013)




Invited professor at Ecole Polytechnique Montreal, Canada, july 2009- Feb. 2010.

Assistant Professor at INSA of Toulouse (2000-2008), France.

Post-doctoral period, Queensland University, AWMC, Brisbane, Australia, july 1999 – January 2000.

Invited PhD, Laval University, Quebec, Canada, Oct.-déc. 1997

PhD in Chemical Engineering, INSA of Toulouse, 1995-1998, France





Biological processes for wastewaters treatment and resources recovery

  • Dynamic modelling of microbial and chemical processes
  • Nitrogen and phosphorus removal and recovery
  • Activated sludge processes, microbial granulation


Recent funding programs

ANR (N2OTrack, 2015-2018), Bank for Investment (VALODIM, 2014-2019), ANR ECOTech (CreativERU, 2010-2014), MidiPy (VALORCAP, 2008-2013). ANR PRECODD (HYBIOX, 2006-2009), ANR ECOTech (PHOSPH’OR, 2010-2014).




-      Process dynamic modelling and control

-      Chemistry and wastewater characterization

-      Wastewater treatment by biological processes




Scientific production

- International publications: 61 SCI-tracked journal papers

- International communications: 55

- International invited conferences: 3

- PhD supervisor: 21

- Post doc supervisor: 7

- Books Chapters: 5

- Patents: 2


Journal Publications

  1. Mathieu Spérandio, Mathieu Pocquet, Lisha Guo, Bing-Jie Ni, Peter A. Vanrolleghem, Zhiguo Yuan: Evaluation of different nitrous oxide production models with four continuous long-term wastewater treatment process data series. Bioprocess and Biosystems Engineering 01/2016;  DOI:10.1007/s00449-015-1532-2
  2. M. Pocquet, Z. Wu, Isabelle Queinnec, M. Spérandio: A two pathway model for N2O emissions by ammonium oxidizing bacteria supported by the NO/N2O variation. Water Research 11/2015; 88. DOI:10.1016/j.watres.2015.11.029
  3. Irene Mozo, Nicolas Lesage, Mathieu Sperandio, Yolaine Bessiere: Impact of sonication on activated sludge properties and consequences on PAH partitioning. The Canadian Journal of Chemical Engineering 11/2015;  DOI:10.1002/cjce.22393
  4. Noureddine LEBAZ, Arnaud COCKX, Mathieu SPERANDIO, Jerome MORCHAIN: Reconstruction of a distribution from a finite number of its moments: A comparative study in the case of depolymerization process. Computers & Chemical Engineering 09/2015; 84. DOI:10.1016/j.compchemeng.2015.09.008
  5. A. B. Bisinella de Faria, M Spérandio, A Ahmadi, L Tiruta-Barna: Evaluation of new alternatives in wastewater treatment plants based on Dynamic Modelling and Life Cycle Assessment (DM-LCA). Water Research 07/2015; 84:99-111. DOI:10.1016/j.watres.2015.06.048
  6. Hélène Hauduc, Imre Takacs, Scott Smith, Anita Szabo, Sudhir Murthy, Glen T. Daigger, Mathieu Spérandio: A Dynamic Physicochemical Model for Chemical Phosphorus Removal. Water Research 04/2015; 2015(73):157-170. DOI:10.1016/j.watres.2014.12.053
  7. Noureddine Lebaz, Jérôme Morchain, Arnaud Cockx, Mathieu Sperandio: Population balance approach for the modeling of enzymatic hydrolysis of cellulose. The Canadian Journal of Chemical Engineering 12/2014; 93(2). DOI:10.1002/cjce.22088
  8. David L Achat, mathieu Spérandio, Marie-Line Daumer, Anne-Cécile santellani, Loïc Prud'Homme, Muhammad Akhtar, Christian Morel: Plant-availability of phosphorus recycled from pig manures and dairy effluents as assessed by isotopic labeling techniques. Geoderma 11/2014; 232-234:24-33. DOI:10.1016/j.geoderma.2014.04.028
  9. Caudan Cédric, Filali Ahlem, Spérandio Mathieu, Girbal-Neuhauser Elisabeth: Multiple EPS interactions involved in the cohesion and structure of aerobic granules.. Chemosphere 08/2014; 117C:262-270. DOI:10.1016/j.chemosphere.2014.07.020
  10. David L. Achat, Marie-Line Daumer, Mathieu Sperandio, Anne-Cécile Santellani, Christian Morel: Solubility and mobility of phosphorus recycled from dairy effluents and pig manures in incubated soils with different characteristics. Nutrient Cycling in Agroecosystems 07/2014; 99(1-3):1-15. DOI:10.1007/s10705-014-9614-0
  11. David L. Achat, Mathieu Sperandio, Marie-Line Daumer, Anne-Cécile Santellani, Loïc Prud'Homme, Muhammad Akhtar, Christian Morel: Plant-availability of phosphorus recycled from pig manures and dairy effluents as assessed by isotopic labeling techniques. Geoderma 01/2014; s 232–234:24–33.
  12. Nicolas Philippe, Anne-Emmanuelle Stricker, Yvan Racault, Alain Husson, Mathieu Sperandio, Peter Vanrolleghem: Modelling the long-term evolution of permeability in a full-scale MBR: Statistical approaches. Desalination 09/2013; 325:7-15. DOI:10.1016/j.desal.2013.04.027
  13. Jean-Marc Choubert, Leiv Rieger, Andrew Shaw, John Copp, Mathieu Spérandio, Kim Sørensen, Sabine Rönner-Holm, Eberhard Morgenroth, Henryk Melcer, Sylvie Gillot: Rethinking wastewater characterisation methods for activated sludge systems - a position paper. Water Science & Technology 06/2013; 67(11):2363-73. DOI:10.2166/wst.2013.158
  14. Mathieu Spérandio, Marc-André Labelle, Abdellah Ramdani, Alain Gadbois, Etienne Paul, Yves Comeau, Peter L Dold: Modelling the degradation of endogenous residue and 'unbiodegradable' influent organic suspended solids to predict sludge production. Water Science & Technology 01/2013; 67(4):789-96. DOI:10.2166/wst.2012.629
  15. N. Philippe, Y. Racault, A.E. Stricker, M. Spérandio, P.A. Vanrolleghem: Modelling the Long-Term Evolution of Permeability in a Full-Scale Municipal MBR: A Multivariate Statistical Modelling Approach. Procedia Engineering 12/2012; 44. DOI:10.1016/j.proeng.2012.08.490
  16. A Mañas, M Spérandio, F Decker, B Biscans: Location and chemical composition of microbially induced phosphorus precipitates in anaerobic and aerobic granular sludge. Environmental Technology 10/2012; 33(19-21):2195-209. DOI:10.1080/09593330.2012.696719
  17. I Mozo, G Lesage, J Yin, Y Bessiere, L Barna, M Sperandio: Dynamic modeling of biodegradation and volatilization of hazardous aromatic substances in aerobic bioreactor. Water Research 07/2012; 46(16):5327-42. DOI:10.1016/j.watres.2012.07.014
  18. Angela Mañas, Mathieu Pocquet, Béatrice Biscans, Mathieu Sperandio: Parameters influencing calcium phosphate precipitation in granular sludge sequencing batch reactor. Chemical Engineering Science 07/2012; 77:165–175. DOI:10.1016/j.ces.2012.01.009
  19. Ahlem Filali, Angéla Ma˜nas, Myriam Mercade, Yolaine Bessière, Béatrice Biscans, Mathieu Spérandio: Stability and performance of two GSBR operated in alternating anoxic/aerobic or anaerobic/aerobic conditions for nutrient removal. Biochemical Engineering Journal 05/2012; 67. DOI:10.1016/j.bej.2012.05.001
  20. Cédric Caudan, Ahlem Filali, Dominique Lefebvre, Mathieu Spérandio, Elisabeth Girbal-Neuhauser: Extracellular polymeric substances (EPS) from aerobic granular sludges: extraction, fractionation, and anionic properties.. Applied biochemistry and biotechnology 03/2012; 166(7):1685-702. DOI:10.1007/s12010-012-9569-z
  21. Ahlem Filali, Yolaine Bessiere, Mathieu Sperandio: Effects of oxygen concentration on the nitrifying activity of an aerobic hybrid granular sludge reactor. Water Science & Technology 01/2012; 65(2):289-95. DOI:10.2166/wst.2012.795
  22. Junfeng Wan, Irene Mozo, Ahlem Filali, Alain Liné, Yolaine Bessière, Mathieu Spérandio: Evolution of bioaggregate strength during aerobic granular sludge formation. Biochemical Engineering Journal 12/2011; 58-59:69-78. DOI:10.1016/j.bej.2011.08.015
  23. I Mozo, M Stricot, N Lesage, M Spérandio: Fate of hazardous aromatic substances in membrane bioreactors. Water Research 10/2011; 45(15):4551-61. DOI:10.1016/j.watres.2011.06.005
  24. Mañas Angela, Biscans Béatrice, Spérandio Mathieu: Biologically induced phosphorus precipitation in aerobic granular sludge process. Water Research 06/2011; 45(12):3776-86. DOI:10.1016/j.watres.2011.04.031
  25. F Delrue, J M Choubert, A E Stricker, M Spérandio, M Mietton-Peuchot, Y Racault: Modelling a full scale membrane bioreactor using Activated Sludge Model No.1: challenges and solutions. Water Science & Technology 11/2010; 62(10):2205-17. DOI:10.2166/wst.2010.383
  26. A Fenu, G Guglielmi, J Jimenez, M Spèrandio, D Saroj, B Lesjean, C Brepols, C Thoeye, I Nopens: Activated sludge model (ASM) based modelling of membrane bioreactor (MBR) processes: a critical review with special regard to MBR specificities.. Water Research 08/2010; 44(15):4272-94. DOI:10.1016/j.watres.2010.06.007
  27. Geoffroy Lesage, Mathieu Sperandio, Ligia Tiruta-Barna: Analysis and modelling of non-equilibrium sorption of aromatic micro-pollutants on GAC with a multi-compartment dynamic model. Chemical Engineering Journal 06/2010; 160(2-160):457-465. DOI:10.1016/j.cej.2010.03.045
  28. M Stricot, A Filali, N Lesage, M Spérandio, C Cabassud: Side-stream membrane bioreactors: Influence of stress generated by hydrodynamics on floc structure, supernatant quality and fouling propensity. Water Research 04/2010; 44(7):2113-24. DOI:10.1016/j.watres.2009.12.021
  29. Junfeng Wan, Yolaine Bessière, Mathieu Spérandio: Alternating anoxic feast/aerobic famine condition for improving granular sludge formation in sequencing batch airlift reactor at reduced aeration rate.. Water Research 09/2009; 43(20):5097-108. DOI:10.1016/j.watres.2009.08.045
  30. Fériel Rezouga, Moktar Hamdi, Mathieu Sperandio: Variability of kinetic parameters due to biomass acclimation: Case of para-nitrophenol biodegradation. Bioresource Technology 06/2009; 100(21):5021-9. DOI:10.1016/j.biortech.2009.05.039
  31. Junfeng Wan, Mathieu Sperandio: Possible role of denitrification on aerobic granular sludge formation in sequencing batch reactor. Chemosphere 04/2009;  DOI:10.1016/j.chemosphere.2008.11.069
  32. Bui Xuan Thanh a, Mathieu Spérandio b, Christelle Guigui b, Roger Ben Aim b, Junfeng Wan b, Chettiyapan Visvanathan: Coupling sequencing batch airlift reactor (SBAR) and membrane filtration: Influence of nitrate removal on sludge characteristics, effluent quality and filterability. Desalination 11/2008; 250(2010):850-854. DOI:10.1016/j.desal.2008.11.055
  33. M. Spérandio, M. C. Espinosa: Modelling an aerobic submerged membrane bioreactor with ASM models on a large range of sludge retention time. Desalination 10/2008;  DOI:10.1016/j.desal.2007.11.040
  34. M Ras, E Girbal-Neuhauser, E Paul, M Spérandio, D Lefebvre: Protein extraction from activated sludge: An analytical approach. Water Research 05/2008; 42(8-9):1867-78. DOI:10.1016/j.watres.2007.11.011
  35. Marie-Line Daumer, Fabrice Béline, Mathieu Spérandio, Christian Morel: Relevance of a perchloric acid extraction scheme to determine mineral and organic phosphorus in swine slurry. Bioresource Technology 04/2008; 99(5):1319-24. DOI:10.1016/j.biortech.2007.02.040
  36. Bui Xuan Thanh, Chettiyappan Visvanathan, Mathieu Spérandio, Roger Ben Aim: Fouling characterization in aerobic granulation coupled baffled membrane separation unit. Journal of Membrane Science 03/2008; 318(2008):334-339. DOI:10.1016/j.memsci.2008.02.058
  37. Nicolas Lesage, Mathieu Sperandio, Corinne Cabassud: Study of a hybrid process: Adsorption on activated carbon/membrane bioreactor for the treatment of an industrial wastewater. Chemical Engineering and Processing 03/2008; 47(3). DOI:10.1016/j.cep.2007.01.021
  38. V. Pambrun, E. Paul, M. Spérandio: Control and modelling of partial nitrification of effluents with high ammonia concentrations in sequencing batch reactor. Chemical Engineering and Processing 03/2008;  DOI:10.1016/j.cep.2007.01.028
  39. M Sperandio, V Pambrun, E Paul: Simultaneous removal of N and P in a SBR with production of valuable compounds: application to concentrated wastewaters. Water Science & Technology 02/2008; 58(4):859-64. DOI:10.2166/wst.2008.345
  40. M.L. Daumer, F. Béline, F. Guiziou, M. Sperandio: Effect of Nitrification on Phosphorus dissolving in a Piggery Effluent treated by a Sequencing Batch Reactor. Biosystems Engineering 04/2007;  DOI:10.1016/j.biosystemseng.2007.01.001
  41. M. L. Daumer, F. Béline, F. Guiziou, M. Sperandio: Influence of pH and Biological Metabolism on Dissolved Phosphorus during Biological Treatment of Piggery Wastewater. Biosystems Engineering 03/2007; 96(3-96):379-386. DOI:10.1016/j.biosystemseng.2006.11.011
  42. Elodie Singlande, Hélène Roux-de Balmann, Xavier Lefevbre, Mathieu Sperandio: Improvement of the treatment of salted liquid waste by integrated electrodialysis upstream biological treatment. Desalination 11/2006; 199(1):64-66. DOI:10.1016/j.desal.2006.03.020
  43. V Pambrun, E Paul, M Spérandio: Modeling the partial nitrification in sequencing batch reactor for biomass adapted to high ammonia concentrations. Biotechnology and Bioengineering 09/2006; 95(1):120-31. DOI:10.1002/bit.21008
  44. Anthony Massé, Mathieu Spérandio, Corinne Cabassud: Comparison of sludge characteristics and performance of a submerged membrane bioreactor and an activated sludge process at high solids retention time.. Water Research 08/2006; 40(12):2405-15. DOI:10.1016/j.watres.2006.04.015
  45. E. Paul, P. Camacho, M. Sperandio, P. Ginestet: Technical and Economical Evaluation of a Thermal, and Two Oxidative Techniques for the Reduction of Excess Sludge Production. Process Safety and Environmental Protection 07/2006; 84(4):247-252. DOI:10.1205/psep.05207
  46. Isabelle Queinnec, Mathieu Spérandio: Simultaneous estimation of sludge biological activity and influent nitrogen load using ORP and DO dynamics.. Bioprocess and Biosystems Engineering 09/2005; 27(5):329-37. DOI:10.1007/s00449-005-0411-7
  47. N Lesage, M Spérandio, C Cabassud: Performances of a hybrid adsorption/submerged membrane biological process for toxic waste removal.. Water Science & Technology 02/2005; 51(6-7):173-80.
  48. M Spérandio, A Masse, M C Espinosa-Bouchot, C Cabassud: Characterization of sludge structure and activity in submerged membrane bioreactor.. Water Science & Technology 02/2005; 52(10-11):401-8.
  49. M Spérandio, I Queinnec: Online estimation of wastewater nitrifiable nitrogen, nitrification and denitrification rates, using ORP and DO dynamics.. Water Science & Technology 02/2004; 49(1):31-8.
  50. V Pambrun, E Paul, M Spérandio: Treatment of nitrogen and phosphorus in highly concentrated effluent in SBR and SBBR processes.. Water Science & Technology 01/2004; 50(6):269-76.
  51. N. Lesage, M. Spérandio, C. Lafforgue, A. Cockx: Calibration And Application Of A 1-D Model For Oxidation Ditches. Chemical Engineering Research And Design 10/2003; 81(9):1259-1264. Doi:10.1205/026387603770866470
  52. P Ginestet, A Maisonnier, M Spérandio: Wastewater COD characterization: biodegradability of physico-chemical fractions.. Water Science & Technology 02/2002; 45(6):89-97.
  53. E Paul, M L Laval, M Sperandio: Excess sludge production and costs due to phosphorus removal.. Environmental Technology 12/2001; 22(11):1363-71. DOI:10.1080/09593332208618195
  54. M Spérandio, V Urbain, P Ginestet, M J Audic, E Paul: Application of COD fractionation by a new combined technique: comparison of various wastewaters and sources of variability.. Water Science & Technology 02/2001; 43(1):181-90.
  55. T Lendormi, C Prévot, F Doppenberg, M Spérandio, H Debellefontaine: Wet oxidation of domestic sludge and process integration: the Mineralis process.. Water Science & Technology 02/2001; 44(10):163-9.
  56. E Anderson, P Ginestet, M Spérandio, E Paul: Influence of pre-treatments and recycled flows on the COD fractions of a wastewater: a case study.. Water Science & Technology 02/2001; 43(11):99-108.
  57. M Mauret, F Ferrand, V Boisdon, M Spérandio, E Paul: Process using DO and ORP signals for biological nitrification and denitrification: validation of a food-processing industry wastewater treatment plant on boosting with pure oxygen.. Water Science & Technology 02/2001; 44(2-3):163-70.
  58. M. Sperandio, Y. Moreau-Le Golvan, D. Poux, X. Lefebvre, E. Paul: Role of Wastewater Sludges in Stabilisation of Municipal Solid Waste Following Co-Disposal in Landfill. Environmental Technology 11/2000; 21(11):1205-1214. DOI:10.1080/09593332108618163
  59. Spérandio Mathieu, Paul Etienne: Estimation of wastewater biodegradable COD fractions by combining respirometric experiments in various So/Xo ratios. Water Research 03/2000; 34(4-34):1233-1246. DOI:10.1016/S0043-1354(99)00241-9
  60. M. Spérandio, V. Urbain, JM Audic, E. Paul: Use of carbon dioxide evolution rate for determining heterotrophic yield and characterising denitrifying biomass. Water Science & Technology 01/1999; 39(1):139-146. DOI:10.1016/S0273-1223(98)00783-5
  61. Mathieu Spérandio, Etienne Paul: Determination of carbon dioxide evolution rate using on-line gas analysis during dynamic biodegradation experiments.. Biotechnology and Bioengineering 02/1997; 53(3):243-52. DOI:10.1002/(SICI)1097-0290(19970205)53:3<243::AID-BIT1>3.0.CO;2-I