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Gianluca CIOCI


Gianluca CIOCI

INRA Research Engineer

Catalysis and Enzyme Molecular Engineering Team (CIMEs)

+33 (0) 561 55 97 68

LISBP / INSA de Toulouse

135 avenue de Rangueil

31077 Toulouse cedex 4 - FRANCE

cioci @ insa-toulouse.fr




INRA Research Engineer at the Catalysis and Enzyme Molecular Engineering Group of the LISBP Toulouse (2013-present)

CNRS Research Engineer at the Immune Response to Pathogens and Altered-self Group, of the Institute for Structural Biology, Grenoble (2012-2013)

Industrial Liaison Scientist at the Industrial Group of the ESRF, Grenoble (2009-2012)

Postdoctoral fellow at the Macromolecular Crystallography Group of the ESRF, Grenoble (2006-2009)

PhD in Structural and Molecular Biology Chemistry in the Molecular Glycobiology Group of CERMAV, Grenoble, (2006)

Laurea Degree in Organic Chemistry at University of Camerino, Italy (2002)



Research Topics

As research engineer of the National Institute for Agronomic Research (INRA), I provide my colleagues at LISBP my expertise in various fields of structural biology and biophysics such as protein production and purification, macromolecular crystallography, scattering techniques (SAXS, DLS, MALS), ITC, DSF, CD, SPR and basic molecular modelling. My main research interest is the study of enzyme molecular mechanisms through the combination of multidisciplinary approaches. I actively work on the structure-activity relationships of various classes of enzymes such as glucansucrases, glycosyltransferases, glycosyl hydrolases, phosphorylases and depolymerases. Within the PICT-ICEO platform, I am the responsible of the protein purification & characterization facility, teaching students and helping researchers on how to produce, purify and characterize proteins.


Selected Publications

Deciphering an Undecided Enzyme: Investigations of the Structural Determinants Involved in the Linkage Specificity of Alternansucrase. Molina M, Moulis C, Monties N, Pizzut-Serin D, Guieysse D, Morel D, Cioci G, and Remaud-Siméon M. ACS Catalysis, 2019, 9 (3), pp 2222–2237


Investigations on the Determinants Responsible for Low Molar Mass Dextran Formation by DSR-M Dextransucrase. Claverie M, Cioci G, Vuillemin M, Monties N, Roblin P, Lippens G, Remaud-Simeon M, Moulis C. ACS Catalysis, 2017, 7 (10), pp 7106–7119


Conferring cellulose-degrading ability to Yarrowia lipolytica to facilitate a consolidated bioprocessing approach. Guo ZP, Duquesne S, Bozonnet S, Cioci G, Nicaud JM, Marty A, O'Donohue MJ.

Biotechnol Biofuels. 2017 May 19;10:132. 


Structural bases for N-glycan processing by mannoside phosphorylase.

Ladevèze S, Cioci G, Roblin P, Mourey L, Tranier S, Potocki-Véronèse G.

Acta Crystallogr D,2015 Jun;71(Pt 6):1335-46


Burkholderia cenocepacia BC2L-C is a super lectin with dual specificity and proinflammatory activity.

Sulák O, Cioci G, Lameignère E, Balloy V, Round A, Gutsche I, Malinovská L, Chignard M, Kosma P, Aubert DF, Marolda CL, Valvano MA, Wimmerová M, Imberty A. PLoS Pathog. 2011 Sep;7(9)


Structures of the tumor necrosis factor alpha inducing protein Tipalpha: a novel virulence factor from Helicobacter pylori. Tosi T, Cioci G, Jouravleva K, Dian C, Terradot L. FEBS Lett. 2009 May 19;583(10

Beta-propeller crystal structure of Psathyrella velutina lectin: an integrin-like fungal protein interacting with monosaccharides and calcium”. Cioci G, Mitchell EP, Chazalet V, Debray H, Oscarson S, Lahmann M, Gautier C, Breton C, Perez S, Imberty A. J Mol Biol. 2006 Apr 14;357(5):1575-91


Structural basis of calcium and galactose recognition by the lectin PA-IL of Pseudomonas aeruginosa.

Cioci G, Mitchell EP, Gautier C, Wimmerová M, Sudakevitz D, Pérez S, Gilboa-Garber N, Imberty A.

FEBS Lett. 2003 Dec 4;555(2):297-301.