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Associate Professor, Ir. Dr.

Toulouse Biotechnology Institute – Université de Toulouse

135, Avenue de Rangueil, F-31077 Toulouse cedex 4

Tél : +33 (0)6 67 86 49 78



N° ORCID: 0000-0002-1490-0152

Google scholar


Current position

  • Associate Professor
  • Co-Head of the research group “Integrated metabolism and dynamics of metabolic systems“ at the TBI



  • Engineering Degree in Biochem. Eng., INSA-TOULOUSE, 1996
  • M.Sc. in Biochem. Eng., INSA-TOULOUSE, 1996
  • Ph.D. in Microbiology-Biotechnology, INSA-TOULOUSE, 2000
  • Habilitation thesis, Toulouse University, 2014


  • Temporary research and teaching assistant, Laboratory of Biotechnology and Bioprocesses, Toulouse, sept 2000 - aug 2001
  • Post doc, Laboratory of  Biotechnology and Bioprocesses, Toulouse, sept 2001 -  march 2002
  • Temporary research and teaching assistant, University of La Rochelle, sept 2002 - august 2003
  • Research fellow, Princeton University, NJ, USA, January 2011 – July 2011
  • Associate Professor, Université Paul Sabatier, Toulouse, 2003


Research domains

  • Microbial metabolism and metabolic engineering  
  • Fluxomics & Metabolomics (13C metabolomics, metabolite profiling)
  • Mass spectrometry and NMR for metabolomics and fluxomics


List of 10 last relevant publications

·      Pierre Millard, Baudoin Delépine, Matthieu Guionnet, Maud Heuillet, Floriant Bellvert and Fabien Létisse. IsoCor: isotope correction for high-resolution MS labeling experiments. Bioinformatics. 2019 Mar 23. pii: btz209. doi: 10.1093.

·      Clément Patacq, Nicolas Chaudet, Fabien Létisse. Absolute quantification of ppGpp and pppGpp by double-spike isotope dilution ion-chromatography-high-resolution mass spectrometry. Anal Chem., 2018 Aug 15. doi: 10.1021/acs.analchem.8b00829.

·      Sinnaeve D, Dinclaux M, Cahoreau E, Millard P, Portais JC, Létisse F, Lippens G. Improved Isotopic Profiling by Pure Shift Heteronuclear 2D J-Resolved NMR Spectroscopy. Anal Chem. 2018 Mar 20;90(6):4025-4031.

·      Heuillet M, Bellvert F, Cahoreau E, Letisse F, Millard P, Portais JC. Methodology for the Validation of Isotopic Analyses by Mass Spectrometry in Stable-Isotope Labeling Experiments. Anal Chem. 2018 Feb 6;90(3):1852-1860.

·      Sylvie Létoffé, Sabina Chalabaev, José Dugay, Franziska Stressmann, Bianca Audrain, Jean-Charles Portais, Fabien Letisse and Jean-Marc Ghigo. Biofilm microenvironment induces a widespread adaptive amino-acid fermentation pathway conferring strong fitness advantage in Escherichia coli. Plos Genetics, 2017 May 13(5):e1006800. doi: 10.1371/journal.pgen.1006800

·      Alberto Rodriguez, Juan A. Martínez, Guillermo Gosset, Jean-Charles Portais, Fabien Létisse and Francisco Bolivar. Plasmid-encoded biosynthetic genes alleviate metabolic disadvantages while increasing glucose conversion to shikimate in an engineered Escherichia coli strain. Biotechnology & Bioengineering, 2017 Feb 10. doi: 10.1002/bit.26264.

·      Brice Enjalbert, Pierre Millard, Mickael Dinclaux, Jean-Charles Portais and Fabien Létisse. Acetate fluxes in Escherichia coli are determined by the thermodynamic control of the Pta-AckA pathway. Scientific Reports, 2017, 7:42135.

·      Stéphanie Heux, Cécila Bergès, Pierre Millard, Jean-Charles Portais and Fabien Létisse. Recent advances in high-throughput 13C-fluxomics. Current Opinion in Biotechnology, 2016, 43:104-109.

·      Pierre Millard, Stéphane Massou, Jean-Charles Portais and Fabien Létisse. Isotopic studies of metabolic systems by mass spectrometry: using Pascal's triangle to produce biological standards with fully controlled labeling patterns. Analytical Chemistry, 2014. Doi: 10.1021/ac502490g.

·      Pierre Millard, Stéphane Massou, Christoph Wittmann, Jean-Charles Portais and Fabien Létisse. Performing a Reliable Sampling of Metabolic Intermediates for Stationary and Instationary 13C-Metabolic Flux Analysis: Practical Guidelines for Escherichia coli. Analytical Biochemistry, 2014, 465:38-49.