CIMEs Skills & Equiment
Molecular Biology/Microbiology
Gene expression
Mutagenesis & Construction of variant libraries
Adaptation of hosts for enzyme screening (prokayota, eukaryota)
Biochemistry/Chemistry
Screening assays at low, medium and high throughput
Enzyme purification
Chemical synthesis
Biophysics
X-ray crystallography
Small angle scattering
Circular Dicroism
SEC-MALLS
ITC Calorimetry
Computer Sciences
Data mining & Sequence analysis (genomic, metagenomic)
Bioinformatics
Multi-scale molecular modelling
Computational Protein Design
Bioprocesses
Enzyme Immobilization / Enzymatic reactors
Screening facilities PICT-ICEO Platform - PICT-ICEO
QPixII (Genetix) : Colony picker (bacteria or yeasts) enabling the inoculation and re-arraying of mini-cultures in microtiterplates (3000 clones / hour)
K2 (KBiosystems) : Multi-application robot allowing colony picking (contrast, color and fluorescence detection), plate replication, gridding on membrane/gelose, and colony re-arraying. Equipped with hotels for microtiterplate storage and a large worktable, the K2 robot provides high-picking and working speeds and excellent user autonomy.
Genesis RSP200 (Tecan) : An integrated liquid handling station equipped with a robotic arm, various incubators and a spectrophotometer (UV/Vis/fluo, 96/384 well plates), and dedicated to the phenotypic screening of variant librairies (up to 10000 clones a week).
Biomek 2000 (Beckman) : Transfer of liquids in microtiterplates under sterile conditions (2000 clones/ hour).
Infors : Microplate incubator-shakers for cell growth and protein expression in liquid medium (10-65°C, up to 96 microplates).
HPLC, GC (Dionex, Agilent) : Enzymatic activity screening via chromatography.
LC/MS, GC/MS (Dionex, Thermo) : Enzymatic activity screening, mixed molecule identification, direct injection or coupling to liquid or gaz chromatography.
Eclipse Fluorimeter (Varian) : Fluorescence spectroscopy, protein-ligand interactions, thermostability study, cuvette or 96 well microtiter plates
Circular dichroism (Jasco) : Assessment of the structural integrity of the recombinant proteins
Protein purification stations : ÄKTAPrime, ÄKTAPurifier, ÄKTAPure, ÄKTAXpress (GE Healthcare) : manual or automated protein purification, multistep purification.
Computing infrastructure & resources
- 12 multi-core workstations dedicated to molecular modelling activities
- Large data storage (>60To)
- Access to High-Performance computing centres
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Midi-Pyrénées Computing mesocentre |
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National computing centres (TGI): TGCC-Curie, IDRIS-Turing, CINES-Occigen |