ICEO

 

High Throughput Screening platform for Directed Evolution of Enzymes (ICEO)

In charge: Sophie BOZONNET (sophie.bozonnet @ insa-toulouse.fr) (INRA Research Engineer)

 

Aim

Protein directed molecular evolution is based on the production of large variant libraries from a gene of interest using random mutagenesis and/or in vitro recombination/DNA shuffling.

 

This highly innovative method allows the efficient improvement and/or modification of the properties of a given protein, due to the generation of random multiple mutations, which could not have been predicted rationally.

 

The size of the variant libraries can reach 106 to 1013. It is thus necessary to use powerful screening tests to be able to retain the variants of interest.

 

This approach involves a fully-robotised HTS platform for the screening of libraries.

 

This platform is operated by the Enzymatic Catlysis and Molecular Engineering Team, of Prof. M. Remaud-Siméon. Key research topics are on optimisation glucansucrases (synthesis of oligosaccharides, polysaccharides and glucoconjugates from sucrose), lipases (ester/amide hydrolysis and synthesis) and enzymes for degradation of lignocellulases.

 

List of equipments

 

  •  Picking robot for bacterial and yeast clones transfer and culture in microplates (QpixII, Genetix);
  •  Sterile liquid transfer robot (Biomek2000, Beckman);
  •  Two microplate agitation systems for cell growth and gene expression (Kühner and Infors);
  •  Liquid and microplate transfer station with incubator and spectrophotometer for phenotype screening (Genesis RSP200, Tecan).

 

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